ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIKITVGVLA LQGAFLEHLE LLKKAAASLG SQQSSPQWEF LEIRTPQELK RCDALVLPGG 

        70         80         90        100        110        120 
ESTAISLVAA RSNLLEPLRD FVKVHRKPTW GTCAGLILLA ESANRTKKGG QELIGGLDVR 

       130        140        150        160        170        180 
VNRNHFGRQT ESFQAPLDLP FLSTSGTPQQ PFPAVFIRAP VVEKILPHHD GIQVDEAKRV 

       190        200        210        220        230        240 
ETVVAPSRQA ESEASRRAMS RDVEVLASLP GRAAHLAVSG TPIRADEETG DIVAVRQGNV 

       250        260        270 
FGTSFHPELT GDERIHAWWL RQVEDSVKRL Q 

References and documentation are available.

Number of amino acids: 271

Molecular weight: 29676.89

Theoretical pI: 7.11

Amino acid composition: 
Ala (A)  27	 10.0%
Arg (R)  21	  7.7%
Asn (N)   5	  1.8%
Asp (D)  11	  4.1%
Cys (C)   2	  0.7%
Gln (Q)  15	  5.5%
Glu (E)  21	  7.7%
Gly (G)  20	  7.4%
His (H)   8	  3.0%
Ile (I)  12	  4.4%
Leu (L)  30	 11.1%
Lys (K)  11	  4.1%
Met (M)   2	  0.7%
Phe (F)  10	  3.7%
Pro (P)  16	  5.9%
Ser (S)  19	  7.0%
Thr (T)  14	  5.2%
Trp (W)   4	  1.5%
Tyr (Y)   0	  0.0%
Val (V)  23	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1315
Hydrogen    H	      2121
Nitrogen    N	       385
Oxygen      O	       389
Sulfur      S	         4

Formula: C1315H2121N385O389S4
Total number of atoms: 4214

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22125
Abs 0.1% (=1 g/l)   0.746, assuming all pairs of Cys residues form cystines


Ext. coefficient    22000
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.01
This classifies the protein as unstable.



Aliphatic index: 95.02

Grand average of hydropathicity (GRAVY): -0.211