ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIKMWRLQIV LVPPSAQDII TFLEARLNTP QSVSPMVQYN EDIITHNNSI NNCSDPSPTS 

        70         80         90        100        110        120 
PSSQNSIQSN RSSDFINYLP NCKKFLHFTD GDNTLLQLSN EILTKFDRLY PNFKESIEIV 

       130        140        150        160        170        180 
SLQDRHGCDL DSEFIIKDVF ENDGVVLVIL KDELDWSRNQ HISLLQLARQ RRRQDNKPST 

       190        200        210        220        230        240 
KSIVTEKRKK ISKEDLSSIS NKDTMHLIAK SSLKNNFINK SRVSTPLMNE ILPLASKYDA 

       250        260        270        280        290        300 
LNKEKCPMPL TSTVVASNVH KDVKDHARAK EGVVTQGSDN NKENIPSSTQ QQKNDGAKRA 

       310        320        330        340        350        360 
ESKDLDLLRN SSEDADYEPA DENSPQISFD SIDTDFQLST TSHTNSDMHI QYSNPSSGAH 

       370        380        390        400        410        420 
SPRKSSLEIK VQNKKGDDLP LNDKDIGENC RRIEAFSDEE DFNETDNDRA DSFINNSKKA 

       430        440        450        460        470        480 
SMGFRDINSD LDSVSFNSDI ENAVQSTQST KNVVSPPFFP EKELNNRLHQ SQGKEALFRL 

       490        500        510        520        530        540 
VEKEFPDKSL GAASSTSHAK DVKIQETIRK LNRFKPTGET KVQKRNSITE PYYGKFGIMK 

       550        560        570        580        590        600 
KDKPKSITSK GVSLETKHFD DPNTIISGEK FAKFGKIKVK RKTDDVGSKV IEFKRKRNMG 

       610        620        630        640        650        660 
NRSLKDIFAN AGKPPNAAST IKVVKLMRDP VDNSKDKVEA TSNSTAQEQE QVSPKLPVMN 

       670        680        690        700        710        720 
STPGKRKNGQ AIPSSLERTP QLKKVKVTRS HSSPSSSSSM SLESSLDSSS SDDSDDDSDS 

       730        740        750        760        770 
RNVQVKKINF KTSHGPAGNS NGKPMLDVDD NEINTKKYQT PKYVESDEDD Q 

References and documentation are available.

Number of amino acids: 771

Molecular weight: 86366.22

Theoretical pI: 7.97

Amino acid composition: 
Ala (A)  31	  4.0%
Arg (R)  34	  4.4%
Asn (N)  61	  7.9%
Asp (D)  68	  8.8%
Cys (C)   5	  0.6%
Gln (Q)  34	  4.4%
Glu (E)  44	  5.7%
Gly (G)  26	  3.4%
His (H)  15	  1.9%
Ile (I)  46	  6.0%
Leu (L)  50	  6.5%
Lys (K)  80	 10.4%
Met (M)  14	  1.8%
Phe (F)  28	  3.6%
Pro (P)  40	  5.2%
Ser (S) 102	 13.2%
Thr (T)  40	  5.2%
Trp (W)   2	  0.3%
Tyr (Y)  10	  1.3%
Val (V)  41	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 112
Total number of positively charged residues (Arg + Lys): 114

Atomic composition:

Carbon      C	      3721
Hydrogen    H	      6002
Nitrogen    N	      1080
Oxygen      O	      1243
Sulfur      S	        19

Formula: C3721H6002N1080O1243S19
Total number of atoms: 12065

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26150
Abs 0.1% (=1 g/l)   0.303, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.300, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.28
This classifies the protein as unstable.



Aliphatic index: 68.00

Grand average of hydropathicity (GRAVY): -0.900