ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIKNYLGRRW LNNPAIQAYV KQNAAVAHST VFQGNLYEYT VMRELSEKLR MTKLRKTGGA 

        70         80         90        100        110        120 
HDGGVDIKGS WPVDDIYWKI SSLMPNLEMA SNIKRTNSQN GFVLKPLKYR IIDHTFEPLK 

       130        140        150        160        170        180 
VLVQCKAFTK SKLSPREFRE LVGTFTSLVS HSQRNKTVCI MCSPHMLTKD TLNLINNITL 

       190        200        210        220        230        240 
PLIYLRVEML KEKTDGHFDL INSGKLINYY ENSYASTLMQ DCKISEWLKL KLYKNSDFNS 


EK 

References and documentation are available.

Number of amino acids: 242

Molecular weight: 27966.50

Theoretical pI: 9.53

Amino acid composition: 
Ala (A)   9	  3.7%
Arg (R)  11	  4.5%
Asn (N)  18	  7.4%
Asp (D)  10	  4.1%
Cys (C)   4	  1.7%
Gln (Q)   7	  2.9%
Glu (E)  12	  5.0%
Gly (G)  11	  4.5%
His (H)   6	  2.5%
Ile (I)  15	  6.2%
Leu (L)  28	 11.6%
Lys (K)  24	  9.9%
Met (M)   9	  3.7%
Phe (F)   8	  3.3%
Pro (P)   8	  3.3%
Ser (S)  19	  7.9%
Thr (T)  15	  6.2%
Trp (W)   4	  1.7%
Tyr (Y)  11	  4.5%
Val (V)  13	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1254
Hydrogen    H	      1998
Nitrogen    N	       340
Oxygen      O	       357
Sulfur      S	        13

Formula: C1254H1998N340O357S13
Total number of atoms: 3962

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38640
Abs 0.1% (=1 g/l)   1.382, assuming all pairs of Cys residues form cystines


Ext. coefficient    38390
Abs 0.1% (=1 g/l)   1.373, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.03
This classifies the protein as stable.



Aliphatic index: 88.60

Grand average of hydropathicity (GRAVY): -0.386