ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIKSEADLSD VIVIRDVWAY NLESEFDLIR GIVEDYPFIS MDTEFPGVIY KADLDVLRRG 

        70         80         90        100        110        120 
NPNYLYNLLK SNVDALSLIQ VGLTLSDADG NLPDLGGQKN RRYIWEFNFR DFDVERDPHA 

       130        140        150        160        170        180 
PDSIELLRRH GIDFERNRRE GVESERFAEL MMSSGLICNE SVSWVTFHSA YDFGYLVKIL 

       190        200        210        220        230        240 
TRRQLPVALR EFLGLLRAFF GDRVYDVKHI MRFCEQRLYG GLDRVARSLE VNRAVGKCHQ 

       250        260        270 
AGSDSLLTWQ AFQRMRDLYF VEDGAEKHAG VLYGLEVF 

References and documentation are available.

Number of amino acids: 278

Molecular weight: 32168.54

Theoretical pI: 5.18

Amino acid composition: 
Ala (A)  16	  5.8%
Arg (R)  26	  9.4%
Asn (N)  11	  4.0%
Asp (D)  24	  8.6%
Cys (C)   3	  1.1%
Gln (Q)   7	  2.5%
Glu (E)  20	  7.2%
Gly (G)  20	  7.2%
His (H)   6	  2.2%
Ile (I)  14	  5.0%
Leu (L)  33	 11.9%
Lys (K)   8	  2.9%
Met (M)   6	  2.2%
Phe (F)  17	  6.1%
Pro (P)   7	  2.5%
Ser (S)  17	  6.1%
Thr (T)   5	  1.8%
Trp (W)   4	  1.4%
Tyr (Y)  12	  4.3%
Val (V)  22	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1445
Hydrogen    H	      2228
Nitrogen    N	       398
Oxygen      O	       419
Sulfur      S	         9

Formula: C1445H2228N398O419S9
Total number of atoms: 4499

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40005
Abs 0.1% (=1 g/l)   1.244, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   1.240, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.82
This classifies the protein as stable.



Aliphatic index: 94.64

Grand average of hydropathicity (GRAVY): -0.229