ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MILKLVHCLV ALTGLIFAKP YQQQQAVLAP SQDVPLRDIH IGDINFIHTT DTHGWLGSHL 

        70         80         90        100        110        120 
SQNDYDADWG DFVAFVDILR EKILRQSRDV IVIDTGDKRD GNGLSDATWP PGLRSSEIFN 

       130        140        150        160        170        180 
MMDYDLLTLG NHELYTAESA ILEYRGTSQS SKFKDKYVCS NVEFIEDDGT RVPFGNKYIT 

       190        200        210        220        230        240 
FETPIMKQRV LALSFLFSFQ RANNRAIVTP PLEEITQKSW FQNMVETNRE EEIDLIIVFG 

       250        260        270        280        290        300 
HLPATDPTER EMHKIHALIR KYYPNTVIQY FGGHTHIRDF VQLDSKSTCL QSGRFAETVG 

       310        320        330        340        350        360 
FLSINMTDPV DAESPIFSRR YIDFNKEAFK YHLSKLGHDS NVPVSTKKGK TISRLVNDLR 

       370        380        390        400        410        420 
HELNLNEKLG YIPQTYYVST RPLNSEENLY HLITHKILPN LIPPKNYEPS MSRFILINTG 

       430        440        450        460        470        480 
SVRYDLYKGP FTKDTEYIVM PFNNDWRFIT VPLVVASRVE TYLNKGPVIA SLGIPSSSHH 

       490        500        510        520        530        540 
KQHFGGFQKC PFINNPNLSE GYTTEDDFGC HGDDTPHNSQ REYDIPNVVQ CKEVKKVQEE 

       550        560        570        580        590        600 
EADPSKMVHV IFYSFMELDI LNAVNSIIND LGLRMENLTT NDCSHYGGDS TKKLLRDYFS 


QF 

References and documentation are available.

Number of amino acids: 602

Molecular weight: 68998.09

Theoretical pI: 5.80

Amino acid composition: 
Ala (A)  21	  3.5%
Arg (R)  28	  4.7%
Asn (N)  36	  6.0%
Asp (D)  42	  7.0%
Cys (C)   7	  1.2%
Gln (Q)  22	  3.7%
Glu (E)  35	  5.8%
Gly (G)  33	  5.5%
His (H)  23	  3.8%
Ile (I)  44	  7.3%
Leu (L)  53	  8.8%
Lys (K)  33	  5.5%
Met (M)  12	  2.0%
Phe (F)  33	  5.5%
Pro (P)  31	  5.1%
Ser (S)  43	  7.1%
Thr (T)  38	  6.3%
Trp (W)   5	  0.8%
Tyr (Y)  26	  4.3%
Val (V)  37	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      3100
Hydrogen    H	      4768
Nitrogen    N	       828
Oxygen      O	       922
Sulfur      S	        19

Formula: C3100H4768N828O922S19
Total number of atoms: 9637

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66615
Abs 0.1% (=1 g/l)   0.965, assuming all pairs of Cys residues form cystines


Ext. coefficient    66240
Abs 0.1% (=1 g/l)   0.960, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.14
This classifies the protein as unstable.



Aliphatic index: 84.15

Grand average of hydropathicity (GRAVY): -0.395