ProtParam
User-provided sequence:
10 20 30 40 50 60
MILRLKDQTL IKINSTRSYL SSLVFRRDSH SQARTKPDHD RRRRGYERDV RIEEKKEHDG
70 80 90 100 110 120
LFLCKSKGQH LLTNTRILDS IVRSSDIRPT DTVLEIGPGT GNLTMKLLEA AQNVVAVELD
130 140 150 160 170 180
KRMVEILRKR VSDHGFADKL TIIQKDVLKT DFPHFDLVVA NIPYNISSPL VAKLVYGSNT
190 200 210 220 230 240
FRSATLLLQK EFSRRLLANP GDSDFNRLAV NVKLVADVKF VMDVSKREFV PPPKVDSSVI
250 260 270 280 290 300
RITPKEIIPD VNVQEWLAFT RTCFGKKNKT LGSMFRQKKK VMELQSLSAG RHGSNVEVMN
310 320 330 340 350 360
QTGGDSDSDV EEDGKDDLLC LDTDASMFKE RVIEILRTNG FEEKRPSKLS HRELLHLLSL
370 380
FNQAGIFFHD ITSLQMDLHE
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 43314.78
Theoretical pI: 9.15
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 1910
Hydrogen H 3111
Nitrogen N 551
Oxygen O 571
Sulfur S 12
Formula: C
1910H
3111N
551O
571S
12
Total number of atoms: 6155
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.267, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.265, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.13
This classifies the protein as unstable.
Aliphatic index: 93.55
Grand average of hydropathicity (GRAVY): -0.407