ProtParam
User-provided sequence:
10 20 30 40 50 60
MIMLRIPTRS YCSPSKLIKG VGLSPLKKSL LSKKIKEDIH PSLPVRTRFA PSPTGFLHLG
70 80 90 100 110 120
SLRTALYNYL LARNTNGQFL LRLEDTDQKR LIEGAEENIY EILKWCNINY DETPIKQSER
130 140 150 160 170 180
KLIYDKYVKI LLSSGKAYRC FCSKERLNDL RHSAMELKPP SMASYDRCCA HLGEEEIKSK
190 200 210 220 230 240
LAQGIPFTVR FKSPERYPTF TDLLHGQINL QPQVNFNDKR YDDLILVKSD KLPTYHLANV
250 260 270 280 290 300
VDDHLMGITH VIRGEEWLPS TPKHIALYNA FGWACPKFIH IPLLTTVGDK KLSKRKGDMS
310 320 330 340 350 360
ISDLKRQGVL PEALINFCAL FGWSPPRDLA SKKHECFSME ELETIFNLNG LTKGNAKVDD
370 380 390 400 410 420
KKLWFFNKHF LQKRILNPST LRELVDDIMP SLESIYNTST ISREKVAKIL LNCGGSLSRI
430 440 450 460 470 480
NDFHDEFYYF FEKPKYNDND AVTKFLSKNE SRHIAHLLKK LGQFQEGTDA QEVESMVETM
490 500 510 520 530
YYENGFSRKV TYQAMRFALA GCHPGAKIAA MIDILGIKES NKRLSEGLQF LQREKK
References and
documentation are available.
Number of amino acids: 536
Molecular weight: 61603.30
Theoretical pI: 9.18
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 80
Atomic composition:
Carbon C 2774
Hydrogen H 4388
Nitrogen N 752
Oxygen O 787
Sulfur S 23
Formula: C
2774H
4388N
752O
787S
23
Total number of atoms: 8724
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57925
Abs 0.1% (=1 g/l) 0.940, assuming all pairs of Cys residues form cystines
Ext. coefficient 57300
Abs 0.1% (=1 g/l) 0.930, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.84
This classifies the protein as unstable.
Aliphatic index: 87.54
Grand average of hydropathicity (GRAVY): -0.418