ProtParam
User-provided sequence:
10 20 30 40 50 60
MIMNSTNTVV YIKVKGRRPQ GFLDPPKFEW NGTKERQLWT MVSNLNYSQD QIDWQNLSKI
70 80 90 100 110 120
FETPEFFLKK RTYKLFAEHL ELLQLQLEKK RDLEKYSNDQ VNEGMSDLIH KYTPTLQNDN
130 140 150 160 170 180
LLNVSASPLT TERQDSEEVE TEVTNEALQH LQTSKILNIH KKTSDSENKP NDKLDKDGIN
190 200 210
KEMECGSSDD DLSSSLSVSK SALEEALMDR LQF
References and
documentation are available.
Number of amino acids: 213
Molecular weight: 24707.68
Theoretical pI: 5.06
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1080
Hydrogen H 1716
Nitrogen N 294
Oxygen O 354
Sulfur S 7
Formula: C
1080H
1716N
294O
354S
7
Total number of atoms: 3451
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 0.969, assuming all pairs of Cys residues form cystines
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 0.969, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.82
This classifies the protein as unstable.
Aliphatic index: 76.85
Grand average of hydropathicity (GRAVY): -0.861