ProtParam
User-provided sequence:
10 20 30 40 50 60
MINGVYYNEI SRDLDISSST QCLRFLKETV IPSLANNGNN STSIQYHGIS KNDNIKKSVN
70 80 90 100 110 120
KLDKQINMAD RSLGLQQVVC IFSYGPHIQK MLSILEIFKK GYIKNNKKIY QWNKLTSFDI
130 140 150
KREGRNELQE ERLKVPILVT LVSDSEIIDL NLHSFTKQ
References and
documentation are available.
Number of amino acids: 158
Molecular weight: 18209.96
Theoretical pI: 9.30
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 809
Hydrogen H 1311
Nitrogen N 223
Oxygen O 243
Sulfur S 5
Formula: C
809H
1311N
223O
243S
5
Total number of atoms: 2591
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 0.800, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.793, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.59
This classifies the protein as unstable.
Aliphatic index: 99.87
Grand average of hydropathicity (GRAVY): -0.434