ProtParam
User-provided sequence:
10 20 30 40 50 60
MINIPTAILC RLGARSSISR SFGTSIVTKS EAKTPIQKFG WEYLLKQRSK NRPIAPHLTV
70 80 90 100 110 120
YQPQLTWMLS GFHRISGCVM AGTLLVGGIG FAVLPFDFTA FVDFIRSWNL PCAVTAVFKY
130 140 150 160 170 180
IIAFPIIFHT LNGIRFLGFD LAKGVNNVGQ IYKSGYLVSG LSAILALAIV FNSCQNKSNK
TA
References and
documentation are available.
Number of amino acids: 182
Molecular weight: 19981.57
Theoretical pI: 10.08
Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 18
Atomic composition:
Carbon C 925
Hydrogen H 1450
Nitrogen N 240
Oxygen O 239
Sulfur S 7
Formula: C
925H
1450N
240O
239S
7
Total number of atoms: 2861
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24200
Abs 0.1% (=1 g/l) 1.211, assuming all pairs of Cys residues form cystines
Ext. coefficient 23950
Abs 0.1% (=1 g/l) 1.199, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.95
This classifies the protein as stable.
Aliphatic index: 104.51
Grand average of hydropathicity (GRAVY): 0.436