ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MINTGRSRNS VLLAHRFLST GGFWRGGTNG TMSRTINNVN PFKLKFIPKT VPAAADSVSP 

        70         80         90        100        110        120 
DSQRPGKKPF KFIVSNQSKS SKASKSPKWS SYAFPSRETI KSHEEAIKKQ NKAIDEQIAA 

       130        140        150        160        170        180 
AVSKNDCSCT EPPKKRKRKL RPRKALITLS PKAIKHLRAL LAQPEPKLIR VSARNRGCSG 

       190        200        210        220        230        240 
LTYDLQYITE PGKFDEVVEQ DGVKIVIDSK ALFSIIGSEM DWIDDKLASK FVFKNPNSKG 

       250 
TCGCGESFMV 

References and documentation are available.

Number of amino acids: 250

Molecular weight: 27674.97

Theoretical pI: 10.04

Amino acid composition: 
Ala (A)  18	  7.2%
Arg (R)  15	  6.0%
Asn (N)  12	  4.8%
Asp (D)  11	  4.4%
Cys (C)   5	  2.0%
Gln (Q)   7	  2.8%
Glu (E)  11	  4.4%
Gly (G)  15	  6.0%
His (H)   3	  1.2%
Ile (I)  17	  6.8%
Leu (L)  15	  6.0%
Lys (K)  30	 12.0%
Met (M)   4	  1.6%
Phe (F)  12	  4.8%
Pro (P)  16	  6.4%
Ser (S)  28	 11.2%
Thr (T)  12	  4.8%
Trp (W)   3	  1.2%
Tyr (Y)   3	  1.2%
Val (V)  13	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1226
Hydrogen    H	      1989
Nitrogen    N	       353
Oxygen      O	       357
Sulfur      S	         9

Formula: C1226H1989N353O357S9
Total number of atoms: 3934

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21220
Abs 0.1% (=1 g/l)   0.767, assuming all pairs of Cys residues form cystines


Ext. coefficient    20970
Abs 0.1% (=1 g/l)   0.758, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.40
This classifies the protein as unstable.



Aliphatic index: 72.20

Grand average of hydropathicity (GRAVY): -0.530