ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIPSRISHKF PLFLRSSLAA PKAAYRFSST IPKPSDQVPD VDAFLNKIGR NCNELKDTFE 

        70         80         90        100        110        120 
NNWNNLFQWD SKILKEKGVN IQQRKYILKQ VHNYRNNRPI HEIKLGKKSF FGGERKRKAF 

       130 
TAKWKAENKQ 

References and documentation are available.

Number of amino acids: 130

Molecular weight: 15291.64

Theoretical pI: 10.29

Amino acid composition: 
Ala (A)   8	  6.2%
Arg (R)   9	  6.9%
Asn (N)  12	  9.2%
Asp (D)   5	  3.8%
Cys (C)   1	  0.8%
Gln (Q)   6	  4.6%
Glu (E)   6	  4.6%
Gly (G)   5	  3.8%
His (H)   3	  2.3%
Ile (I)   9	  6.9%
Leu (L)   9	  6.9%
Lys (K)  18	 13.8%
Met (M)   1	  0.8%
Phe (F)   9	  6.9%
Pro (P)   7	  5.4%
Ser (S)   9	  6.9%
Thr (T)   3	  2.3%
Trp (W)   3	  2.3%
Tyr (Y)   3	  2.3%
Val (V)   4	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	       693
Hydrogen    H	      1090
Nitrogen    N	       202
Oxygen      O	       186
Sulfur      S	         2

Formula: C693H1090N202O186S2
Total number of atoms: 2173

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20970
Abs 0.1% (=1 g/l)   1.371, assuming all pairs of Cys residues form cystines


Ext. coefficient    20970
Abs 0.1% (=1 g/l)   1.371, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.88
This classifies the protein as unstable.



Aliphatic index: 69.08

Grand average of hydropathicity (GRAVY): -0.888