ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIQDPLVYLD ISIDKKPIGR IVCKLFREKA PKTTENFYKL CAGDVKSPLK DQQYLSYKGN 

        70         80         90        100        110        120 
GFHRVVKNFM IQAGDIVFGT QKDSSSSSVG KGGCSIYADK EEVKTDDESF CYGNFEDENL 

       130        140        150        160        170        180 
GEFVEPFTLG MANLGSPNTN NSQFFITTYA APHLNGKHSI FGQVVHGKSV VRTIENCRVD 

       190        200        210        220        230        240 
SDGVPESDVR ISDCGVWEKT MGVPLYNASN DQIGGDVYEE YPDDDTHFGD DDFGKALEAA 

       250        260        270        280        290        300 
NIIKESGTLL FKKKDYSNAF FKYRKSLNYI NEYMPEPDVD KERNIQFINL KMKIYLNLSL 

       310        320        330        340        350        360 
VLFNLERYDD AIMYATYLLE MDNVPNRDQA KAYYRRGNSY LKKKRLDEAL QDYIFCKEKN 

       370        380        390 
PDDEVIEQRI EYVNRLIEEN KEKTRKNISK FFS 

References and documentation are available.

Number of amino acids: 393

Molecular weight: 45133.83

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  17	  4.3%
Arg (R)  16	  4.1%
Asn (N)  28	  7.1%
Asp (D)  34	  8.7%
Cys (C)   7	  1.8%
Gln (Q)  12	  3.1%
Glu (E)  29	  7.4%
Gly (G)  25	  6.4%
His (H)   5	  1.3%
Ile (I)  25	  6.4%
Leu (L)  27	  6.9%
Lys (K)  37	  9.4%
Met (M)   8	  2.0%
Phe (F)  22	  5.6%
Pro (P)  14	  3.6%
Ser (S)  25	  6.4%
Thr (T)  14	  3.6%
Trp (W)   1	  0.3%
Tyr (Y)  23	  5.9%
Val (V)  24	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2012
Hydrogen    H	      3101
Nitrogen    N	       529
Oxygen      O	       622
Sulfur      S	        15

Formula: C2012H3101N529O622S15
Total number of atoms: 6279

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40145
Abs 0.1% (=1 g/l)   0.889, assuming all pairs of Cys residues form cystines


Ext. coefficient    39770
Abs 0.1% (=1 g/l)   0.881, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.79
This classifies the protein as stable.



Aliphatic index: 73.64

Grand average of hydropathicity (GRAVY): -0.623