ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIQKMSPSLR RPSTRSSSGS SNIPQSPSVR STSSFSNLTR NSIRSTSNSG SQSISASSTR 

        70         80         90        100        110        120 
SNSPLRSVSA KSDPFLHPGR IRIRRSDSIN NNSRKNDTYT GSITVTIRPK PRSVGTSRDH 

       130        140        150        160        170        180 
VGLKSPRYSQ PRSNSHHGSN TFVRDPWFIT NDKTIVHEEI GEFKFDHVFA SHCTNLEVYE 

       190        200        210        220        230        240 
RTSKPMIDKL LMGFNATIFA YGMTGSGKTF TMSGNEQELG LIPLSVSYLF TNIMEQSMNG 

       250        260        270        280        290        300 
DKKFDVIISY LEIYNERIYD LLESGLEESG SRISTPSRLY MSKSNSNGLG VELKIRDDSQ 

       310        320        330        340        350        360 
YGVKVIGLTE RRCESSEELL RWIAVGDKSR KIGETDYNAR SSRSHAIVLI RLTSTNVKNG 

       370        380        390        400        410        420 
TSRSSTLSLC DLAGSERATG QQERRKEGSF INKSLLALGT VISKLSADKM NSVGSNIPSP 

       430        440        450        460        470        480 
SASGSSSSSG NATNNGTSPS NHIPYRDSKL TRLLQPALSG DSIVTTICTV DTRNDAAAET 

       490        500        510        520        530        540 
MNTLRFASRA KNVALHVSKK SIISNGNNDG DKDRTIELLR RQLEEQRRMI SELKNRSNIG 

       550        560        570        580        590        600 
EPLTKSSNES TYKDIKATGN DGDPNLALMR AENRVLKYKL ENCEKLLDKD VVDLQDSEIM 

       610        620        630        640        650        660 
EIVEMLPFEV GTLLETKFQG LESQIRQYRK YTQKLEDKIM ALEKSGHTAM SLTGCDGTEV 

       670        680        690        700 
IELQKMLERK DKMIEALQSA KRLRDRALKP LINTQQSPHP VVDNDK 

References and documentation are available.

Number of amino acids: 706

Molecular weight: 78378.18

Theoretical pI: 9.44

Amino acid composition: 
Ala (A)  29	  4.1%
Arg (R)  54	  7.6%
Asn (N)  45	  6.4%
Asp (D)  37	  5.2%
Cys (C)   6	  0.8%
Gln (Q)  21	  3.0%
Glu (E)  44	  6.2%
Gly (G)  43	  6.1%
His (H)  12	  1.7%
Ile (I)  46	  6.5%
Leu (L)  62	  8.8%
Lys (K)  46	  6.5%
Met (M)  19	  2.7%
Phe (F)  16	  2.3%
Pro (P)  26	  3.7%
Ser (S) 102	 14.4%
Thr (T)  49	  6.9%
Trp (W)   2	  0.3%
Tyr (Y)  16	  2.3%
Val (V)  31	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      3356
Hydrogen    H	      5502
Nitrogen    N	      1006
Oxygen      O	      1102
Sulfur      S	        25

Formula: C3356H5502N1006O1102S25
Total number of atoms: 10991

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35215
Abs 0.1% (=1 g/l)   0.449, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.445, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.82
This classifies the protein as unstable.



Aliphatic index: 76.50

Grand average of hydropathicity (GRAVY): -0.640