ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIQSDEDNLD SSETTASTSY SGTSSVSSRL QLRTSLFFEN LNGAHGNPDA ETEMATVAYE 

        70         80         90        100        110        120 
TTSRGQGFAV YINNERFSQI MGASTSSSSS SNSSSITQFH DTQDNNIPSN TTVRPTSLRR 

       130        140        150        160        170        180 
DNEDTVPLRN VTPSQNAAVR PERAVNSPSS QRLSCALTIS TSVLMGEDVE GSPIEQEHSR 

       190        200        210        220        230        240 
VVSSLYSSLA NRGNDESKNG TPPRPTSIEP NETTEHSFFS YHYDDTLEPD VEEAVRLTKN 

       250        260        270        280        290        300 
KTSNVNFISS TGSKGEGETE DEVIDQYEPV NESKFIPHKL KIPEKAGSIK SSTSDDSHSP 

       310        320        330        340        350        360 
GAPGTSARKI KIPQSPSLIG NILIPSHNSD SSNESSPKDH IGHNNEEKFS SKSTRKPSTS 

       370        380        390        400        410        420 
LEEEGPPIGL PSIPVLRSVS GPSKWTKTPL RLESGNSTKS DPFSRYEGHK TPSPLTKMNK 

       430        440        450        460        470        480 
KKNKTLPEHG QPLVLAPIKS QSSESDTGQN SIIEKPARSI RRKQQEKTDN RKEDRHDAEN 

       490        500        510        520        530        540 
IDLEARMPIQ HIDTASIHSF DSGQNGFRDV YSIENIIVIL LCCSIVPPLF FIIGCSSRRK 

       550        560        570        580        590        600 
LVSDYRLMRL LMNKEHRAAL LQGFIWDVDL RWFRMFCLIL GAAETVIVMA GIAIGFGVGI 


TRE 

References and documentation are available.

Number of amino acids: 603

Molecular weight: 66288.51

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  27	  4.5%
Arg (R)  35	  5.8%
Asn (N)  36	  6.0%
Asp (D)  31	  5.1%
Cys (C)   5	  0.8%
Gln (Q)  19	  3.2%
Glu (E)  46	  7.6%
Gly (G)  34	  5.6%
His (H)  16	  2.7%
Ile (I)  42	  7.0%
Leu (L)  39	  6.5%
Lys (K)  31	  5.1%
Met (M)  10	  1.7%
Phe (F)  19	  3.2%
Pro (P)  39	  6.5%
Ser (S)  90	 14.9%
Thr (T)  43	  7.1%
Trp (W)   3	  0.5%
Tyr (Y)  10	  1.7%
Val (V)  28	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2856
Hydrogen    H	      4561
Nitrogen    N	       829
Oxygen      O	       956
Sulfur      S	        15

Formula: C2856H4561N829O956S15
Total number of atoms: 9217

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31650
Abs 0.1% (=1 g/l)   0.477, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.474, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.42
This classifies the protein as unstable.



Aliphatic index: 70.33

Grand average of hydropathicity (GRAVY): -0.659