ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIQTPRELLN PRYTYHTIFS DVCKTRFNHL VTRLFFICSI IQTVVISLLA LPHSPLWELA 

        70         80         90        100        110        120 
LAFIPNILAL NLVSLLIIVT RKNYMHVKNF GFANSLTFIL GQLLSVKFLV YQGVYSMGSI 

       130        140        150        160        170        180 
LLSFVLGVVF GRGGSGWKPY YKLFIWLVVP TIYNLQHHVT DADKLSFNCE NFFQAPQDYV 

       190        200        210        220        230        240 
LERVKRIMEK SVILSVISMF VLPIFTTVFF SRQKSGLFDS FTNGVLAVTN LLIISCIIFI 

       250        260        270        280        290        300 
TFEFINIAFD AHMSIGCLHK GKLISNLSST PMETLLSGLS ADKPFTRLTA YQELAYRATS 

       310        320        330        340        350        360 
LDPSLRAPIY HSKFRSSSGN TWSLILNECL KTIQINNEKV VQYLRSVQDL GGSATARHKK 

       370        380        390        400        410        420 
KVENLDYMYE NGKLTSANER LFGNRPSMMA PLRDNGLLDE SPNRLRVRTD DSVLLNRGNK 

       430        440        450        460        470        480 
KRHRSSYYDN DLDETTQTFN GSIFTHETTF MTAMRLMLKK LKNSIMSFIF PSYAERQSSD 

       490        500        510        520        530        540 
ESDNYRLLPN GSNKAQISII DIWSISKKRQ AEKLVPLPIC HANSVVALTG LLIRSKTEDP 

       550        560        570        580        590        600 
KGGIIASVGD ILKTLERSIC ALGEFADWDP ESMAYTAFQT QRTAQDRVQQ DSEDEDSMKD 

       610        620        630        640        650 
TTDMISVLYQ LSTSAFMEIV LEYNVALNDV YLDADVAKLA NWFLEVYASG NPNAT 

References and documentation are available.

Number of amino acids: 655

Molecular weight: 74133.54

Theoretical pI: 8.55

Amino acid composition: 
Ala (A)  38	  5.8%
Arg (R)  32	  4.9%
Asn (N)  38	  5.8%
Asp (D)  33	  5.0%
Cys (C)   8	  1.2%
Gln (Q)  22	  3.4%
Glu (E)  28	  4.3%
Gly (G)  29	  4.4%
His (H)  13	  2.0%
Ile (I)  47	  7.2%
Leu (L)  79	 12.1%
Lys (K)  34	  5.2%
Met (M)  18	  2.7%
Phe (F)  37	  5.6%
Pro (P)  23	  3.5%
Ser (S)  62	  9.5%
Thr (T)  42	  6.4%
Trp (W)   7	  1.1%
Tyr (Y)  24	  3.7%
Val (V)  41	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      3350
Hydrogen    H	      5268
Nitrogen    N	       878
Oxygen      O	       966
Sulfur      S	        26

Formula: C3350H5268N878O966S26
Total number of atoms: 10488

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74760
Abs 0.1% (=1 g/l)   1.008, assuming all pairs of Cys residues form cystines


Ext. coefficient    74260
Abs 0.1% (=1 g/l)   1.002, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.57
This classifies the protein as stable.



Aliphatic index: 98.98

Grand average of hydropathicity (GRAVY): 0.005