ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIRLAQQTQV LKGKPPNQFV PHPTKNSLTH PMKFNGTIAM EHHEHNYAIP YTPATFNNPA 

        70         80         90        100        110        120 
LATYQVSPAN HFVPHFGGNI GANNNNHLAQ NNSNNSNNHH NNNRNHHHNN NRNHHQNNHN 

       130        140        150        160        170        180 
HSKYNNSNQG NSISPDSPWF HKVCAFEDCV SQTLYMSQTP RRQNMKHHSE HPNSNANPLF 

       190        200        210        220        230        240 
WDSIGRAMGL YHDLLTTPEL NSDRVSKLVH LLHNGLRANR NQLTRMNKKP DYDSQSFHKE 

       250        260        270        280        290        300 
MTNYLCKSLR EISEDVLNGK VELNEYGAMH LITAFKELLL FEEAVDIWKA AINGQNTYTS 

       310        320        330        340        350        360 
NIFLNPRVVG VILPILYDNG VSYPEIQALY EKSSSMINYF HPNLSVGMIR ASLSASENDM 

       370        380        390        400        410        420 
ALKLFQKLCQ ESTEMKYGYL IETHLSFIGE CKDLNVAQTF FDKALNDEMP YKIDLQVSYV 

       430        440        450        460        470        480 
KSFLRNIWSQ TRDFNHIYQI WYKSSLHYGR NVNHGISSSL NDTFFDIFFE NYAVDKMQGF 

       490        500        510        520        530        540 
QTLQNIIQTY NNIKHIDEPF FNIILAKCTV WHDRSILEYI DKSYEAYHIP KTIVAYRILL 

       550        560        570        580        590        600 
KSMGSVDDAS NAEILQRWMD LIRKSDEIGQ RFIANADWAA LRDATVTWTQ NDRDSKKSNM 

       610        620        630        640        650        660 
NSTQISRTAT PSPSLTPMDT PAPEHLFNNP QNPMDFYSHP ALQAATASGA FDEFAAEAAS 

       670        680        690        700        710        720 
SSIPVDGRMV LYLKIVKRYS PYCRDSRQLA RLTTGTAVKY SVLQEVLNQF QTLIVNDIPI 

       730 
PELHNLKPTC V 

References and documentation are available.

Number of amino acids: 731

Molecular weight: 83761.20

Theoretical pI: 7.10

Amino acid composition: 
Ala (A)  48	  6.6%
Arg (R)  29	  4.0%
Asn (N)  76	 10.4%
Asp (D)  36	  4.9%
Cys (C)   8	  1.1%
Gln (Q)  35	  4.8%
Glu (E)  32	  4.4%
Gly (G)  25	  3.4%
His (H)  37	  5.1%
Ile (I)  45	  6.2%
Leu (L)  61	  8.3%
Lys (K)  37	  5.1%
Met (M)  21	  2.9%
Phe (F)  32	  4.4%
Pro (P)  37	  5.1%
Ser (S)  59	  8.1%
Thr (T)  39	  5.3%
Trp (W)   9	  1.2%
Tyr (Y)  32	  4.4%
Val (V)  33	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      3718
Hydrogen    H	      5693
Nitrogen    N	      1049
Oxygen      O	      1109
Sulfur      S	        29

Formula: C3718H5693N1049O1109S29
Total number of atoms: 11598

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    97680
Abs 0.1% (=1 g/l)   1.166, assuming all pairs of Cys residues form cystines


Ext. coefficient    97180
Abs 0.1% (=1 g/l)   1.160, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.71
This classifies the protein as unstable.



Aliphatic index: 76.21

Grand average of hydropathicity (GRAVY): -0.553