ProtParam
User-provided sequence:
10 20 30 40 50 60
MIRQIILIVS LIGISNAAYQ CKDNNGSNVD WFVFYKLPHL WNHPDNVPIS NGTGFLYFDV
70 80 90 100 110 120
NNKNWKLMPQ GMDVENNAVY YTLQQYYNSN MNTTFSYMYN DEWPDSTIWS NSSGHAKGVT
130 140 150 160 170 180
VFDQYTGFWM IHSIPKFPSK DMFRFPSNAH YYGQMGICIS YNTVSLATIA QQLFYYNTFT
190 200 210 220 230 240
YQFNLPQSFA NQFPVLSQLK NKEYNKSPPL TSTKVLKSLG GQHFRHFAKT GEWGKDLYSD
250 260 270 280 290 300
FVGPTLKSSI KVETWNHQSG DEYNLPSVCD PNHVQSTMSA KYIRLPYAID YSSYEDHSKF
310 320 330 340 350 360
VVAYSESSSK PPIPYVCIGD INRQSHQIHR GGGTMCIYDQ ETYFQFANII SETVPCTKAT
370
AEKATLTVLL IAIITFFK
References and
documentation are available.
Number of amino acids: 378
Molecular weight: 43347.93
Theoretical pI: 7.17
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1977
Hydrogen H 2925
Nitrogen N 499
Oxygen O 572
Sulfur S 16
Formula: C
1977H
2925N
499O
572S
16
Total number of atoms: 5989
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 84605
Abs 0.1% (=1 g/l) 1.952, assuming all pairs of Cys residues form cystines
Ext. coefficient 84230
Abs 0.1% (=1 g/l) 1.943, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.24
This classifies the protein as unstable.
Aliphatic index: 70.13
Grand average of hydropathicity (GRAVY): -0.348