ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIRTVKPKNA RAKRALVKRE AKLVENVKQA LFIPGQSCNK NLHDIMVDLS ALKKPDMKRF 

        70         80         90        100        110        120 
NRKNDIHPFE DMSPLEFFSE KNDCSLMVLM TSSKKRKNNM TFIRTFGYKI YDMIELMVAD 

       130        140        150        160        170        180 
NFKLLSDFKK LTFTVGLKPM FTFQGAAFDT HPVYKQIKSL FLDFFRGEST DLQDVAGLQH 

       190        200        210        220        230        240 
VISMTIQGDF QDGEPLPNVL FRVYKLKSYK SDQGGKRLPR IELVEIGPRL DFKIGRIHTP 

       250        260        270        280        290        300 
SPDMVTEAHK KPKQLEMKTK KNVELDIMGD KLGRIHMGKQ DLGKLQTRKM KGLKSKFDQG 

       310        320        330        340 
TEEGDGEVDE DYEDEASYSD DGQEYEEEFV SATDIEPSAK RQKK 

References and documentation are available.

Number of amino acids: 344

Molecular weight: 39598.69

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  14	  4.1%
Arg (R)  17	  4.9%
Asn (N)  12	  3.5%
Asp (D)  29	  8.4%
Cys (C)   2	  0.6%
Gln (Q)  15	  4.4%
Glu (E)  24	  7.0%
Gly (G)  21	  6.1%
His (H)   7	  2.0%
Ile (I)  17	  4.9%
Leu (L)  30	  8.7%
Lys (K)  43	 12.5%
Met (M)  16	  4.7%
Phe (F)  21	  6.1%
Pro (P)  15	  4.4%
Ser (S)  19	  5.5%
Thr (T)  16	  4.7%
Trp (W)   0	  0.0%
Tyr (Y)   8	  2.3%
Val (V)  18	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      1760
Hydrogen    H	      2815
Nitrogen    N	       479
Oxygen      O	       521
Sulfur      S	        18

Formula: C1760H2815N479O521S18
Total number of atoms: 5593

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12045
Abs 0.1% (=1 g/l)   0.304, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.301, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.07
This classifies the protein as stable.



Aliphatic index: 72.53

Grand average of hydropathicity (GRAVY): -0.669