ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MISPEQEEVN MVLDRHVRQN IKDLMHEAHV HASLLNNEGR DRFHSDSSLD QDSLHAVVGN 

        70         80         90        100        110        120 
DLSTEQGANQ VQNYHDMMVD SEHHVDINGS LRKRRGNLPK SSVKILKRWL YEHRYNAYPS 

       130        140        150        160        170        180 
DAEKFTLSQE ANLTVLQVCN WFINARRRIL PEMIRREGND PLHFTISRRG KKVSPNCSRS 

       190        200        210        220        230        240 
SALGANLTGP NPAHGSPASE VVVGATEEVD GAGEIHEGIA NVLTNFEQYV QGPNGQMVKM 

       250        260        270        280        290        300 
EPEYEDSVIY RSEGEESAQG YESCGPNSEE EVRFETSHDW QSVIKTVFGT EEVSTSAGNN 

       310        320        330        340        350        360 
PGTSGSKASV QNTAIWNRNQ TAKRDVNQQL TDFESELNII QASEIQTIDP TNSNQQDIGD 

       370        380        390        400        410        420 
NLQADEVFTG AEAEAGQSQL SALSQGTSSD EEGKYKCLYY LVETAMAVRQ NDDVQDDDFV 


YMGD 

References and documentation are available.

Number of amino acids: 424

Molecular weight: 47226.55

Theoretical pI: 4.73

Amino acid composition: 
Ala (A)  28	  6.6%
Arg (R)  22	  5.2%
Asn (N)  32	  7.5%
Asp (D)  28	  6.6%
Cys (C)   4	  0.9%
Gln (Q)  27	  6.4%
Glu (E)  40	  9.4%
Gly (G)  29	  6.8%
His (H)  14	  3.3%
Ile (I)  18	  4.2%
Leu (L)  26	  6.1%
Lys (K)  14	  3.3%
Met (M)  10	  2.4%
Phe (F)  10	  2.4%
Pro (P)  14	  3.3%
Ser (S)  40	  9.4%
Thr (T)  20	  4.7%
Trp (W)   4	  0.9%
Tyr (Y)  12	  2.8%
Val (V)  32	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      2015
Hydrogen    H	      3145
Nitrogen    N	       595
Oxygen      O	       692
Sulfur      S	        14

Formula: C2015H3145N595O692S14
Total number of atoms: 6461

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40130
Abs 0.1% (=1 g/l)   0.850, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.844, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.15
This classifies the protein as unstable.



Aliphatic index: 68.96

Grand average of hydropathicity (GRAVY): -0.756