ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MISPEQEEVN MVLDRHVRQN IQDMMHEAHV QASLLENEGR GRFHSDSSLD QDSLHADVIV 

        70         80         90        100        110        120 
EEDQSTEHGA NQVQNYHDMM VDSEHHIDIN GSLRKRRGNL PKTSVKILKR WLYEHRYNAY 

       130        140        150        160        170        180 
PSDAEKFTLS QEANLTVLQV CNWFINARRR ILPEMIRREG NDPLHFTISR RGKKVSPNCS 

       190        200        210        220        230        240 
RSSALGANLT GPNPAHGSPA SEVVVGATEE VDGAGEIHEG IANVLTNFEQ YVQGPNGQMV 

       250        260        270        280        290        300 
KMEPEYEDSV IYRSEGEESA QGYESCGPNS EEEVRFETSH DWQSVIKTVF GTEEVSTSAG 

       310        320        330        340        350        360 
NNPGTSGSKG SVQNTAIWNR NQTAKRDVNQ QLTDFESELN RIQASEIQTI DPTNSNQQDI 

       370        380        390        400        410        420 
GDNLQADEVF TGAEAEAGQS QLSAMSQGTS PDERAKYKCL YYLVETAMAV RQNDDVQDDD 


FVYMGD 

References and documentation are available.

Number of amino acids: 426

Molecular weight: 47658.03

Theoretical pI: 4.72

Amino acid composition: 
Ala (A)  28	  6.6%
Arg (R)  24	  5.6%
Asn (N)  30	  7.0%
Asp (D)  28	  6.6%
Cys (C)   4	  0.9%
Gln (Q)  29	  6.8%
Glu (E)  42	  9.9%
Gly (G)  29	  6.8%
His (H)  14	  3.3%
Ile (I)  19	  4.5%
Leu (L)  23	  5.4%
Lys (K)  13	  3.1%
Met (M)  12	  2.8%
Phe (F)  10	  2.3%
Pro (P)  15	  3.5%
Ser (S)  38	  8.9%
Thr (T)  21	  4.9%
Trp (W)   4	  0.9%
Tyr (Y)  12	  2.8%
Val (V)  31	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      2029
Hydrogen    H	      3166
Nitrogen    N	       602
Oxygen      O	       697
Sulfur      S	        16

Formula: C2029H3166N602O697S16
Total number of atoms: 6510

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40130
Abs 0.1% (=1 g/l)   0.842, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.837, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.45
This classifies the protein as unstable.



Aliphatic index: 66.13

Grand average of hydropathicity (GRAVY): -0.800