ProtParam
User-provided sequence:
10 20 30 40 50 60
MISPSKKRTI LSSKNINQKP RAVVKGNELR SPSKRRSQID TDYALRRSPI KTIQISKAAQ
70 80 90 100 110 120
FMLYEETAEE RNIAVHRHNE IYNNNNSVSN ENNPSQVKEN LSPAKICPYE RAFLREGGRI
130 140 150 160 170 180
ALKDLSVDEF KGYIQDPLTD ETIPLTLPLG DKKISLPSFI TPPRNSKISI FFTSKHQGQN
190 200
PETKISRSTD DVSEKKVVRK LSFHVYEDE
References and
documentation are available.
Number of amino acids: 209
Molecular weight: 23870.06
Theoretical pI: 9.58
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1048
Hydrogen H 1701
Nitrogen N 305
Oxygen O 325
Sulfur S 3
Formula: C
1048H
1701N
305O
325S
3
Total number of atoms: 3382
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.375, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.375, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.10
This classifies the protein as unstable.
Aliphatic index: 77.89
Grand average of hydropathicity (GRAVY): -0.814