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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MISSCVTRCF GRGKCLPGPA TASIYQTIRC ISTNSNKAAE APIFPKLEDV KMHELIGNNN

70 80 90 100 110 120
FGKKTYYVER SRTGNLPVYS AYKNGGNKII TEIRKIEGDV IQLRNDLQEQ LPFIPKKSWS

130 140
VVMQSKKIII KGNAVEAVKR VLTKKF


References and documentation are available.

Number of amino acids: 146 Molecular weight: 16380.19 Theoretical pI: 9.87
Amino acid composition: 
Ala (A) 8 5.5% Arg (R) 8 5.5% Asn (N) 10 6.8% Asp (D) 3 2.1% Cys (C) 4 2.7% Gln (Q) 5 3.4% Glu (E) 8 5.5% Gly (G) 10 6.8% His (H) 1 0.7% Ile (I) 15 10.3% Leu (L) 8 5.5% Lys (K) 17 11.6% Met (M) 3 2.1% Phe (F) 5 3.4% Pro (P) 7 4.8% Ser (S) 10 6.8% Thr (T) 8 5.5% Trp (W) 1 0.7% Tyr (Y) 5 3.4% Val (V) 10 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 11 Total number of positively charged residues (Arg + Lys): 25 Atomic composition: Carbon C 730 Hydrogen H 1195 Nitrogen N 205 Oxygen O 207 Sulfur S 7 Formula: C730H1195N205O207S7 Total number of atoms: 2344 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13200 Abs 0.1% (=1 g/l) 0.806, assuming all pairs of Cys residues form cystines Ext. coefficient 12950 Abs 0.1% (=1 g/l) 0.791, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.07 This classifies the protein as unstable. Aliphatic index: 86.78 Grand average of hydropathicity (GRAVY): -0.334