ProtParam
User-provided sequence:
10 20 30 40 50 60
MISSCVTRCF GRGKCLPGPA TASIYQTIRC ISTNSNKAAE APIFPKLEDV KMHELIGNNN
70 80 90 100 110 120
FGKKTYYVER SRTGNLPVYS AYKNGGNKII TEIRKIEGDV IQLRNDLQEQ LPFIPKKSWS
130 140
VVMQSKKIII KGNAVEAVKR VLTKKF
References and
documentation are available.
Number of amino acids: 146
Molecular weight: 16380.19
Theoretical pI: 9.87
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 730
Hydrogen H 1195
Nitrogen N 205
Oxygen O 207
Sulfur S 7
Formula: C
730H
1195N
205O
207S
7
Total number of atoms: 2344
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13200
Abs 0.1% (=1 g/l) 0.806, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.791, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.07
This classifies the protein as unstable.
Aliphatic index: 86.78
Grand average of hydropathicity (GRAVY): -0.334