ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MISSELTNDQ IIDLISDYKN FKRIIEASVP EDDRRRNQNR QNRNKDLTKL SNVQFWQLTT 

        70         80         90        100        110        120 
DVNDELMKRL TDSGADASLN DLDLKRGKAQ SKLSRLKDAK FHKLILDIFT EIERRNLHHL 

       130        140        150        160        170        180 
DMGTHNNGLD EGDLNFYLND TLFESFKIND DFMSVNGIIS IEVFRELKTQ FTLYFQNTLH 

       190        200        210        220        230        240 
RIDPVDTTTT RLPILLETII KIAKLIGDLL PVLSSVSLQS SLENEIVYLK SALSHAITST 

       250        260        270        280        290        300 
RYFLTYGDLI PRIVAQSSIS EVIFAFCNIA QIVKIKSTSR DDISRNEGEL SDIEAGMKPL 

       310        320        330        340        350        360 
KIIEKVKNEK NGKDISSLGD SGSTAVSFPS SGKPITKKSD MPVVVASPSI SIIEKSESSI 

       370        380        390        400        410        420 
RESGKVRNNT SGETNLASVS PLKNTKNSSR ITSEPSPREG LPLKVVSNSR SPSPQGNTLP 

       430        440        450        460        470        480 
LIGKFRQDYQ ASPPKKVITK PVAETAKPYA NIPPAADVLY SPTVTKMRKF REKVQKFAPN 

       490        500        510        520        530        540 
SGLGLRISTS EENLNNSDVN STTHNANINN LVEFVESKSM VVLPMAQGIL NDVQASKSKL 

       550        560        570        580        590        600 
FKSARSVSRL CQNSIEIIPI LESVIDMTTK AMVQKSFKLD LGEHCKEIIE RLTDCSQKLS 

       610        620        630        640        650        660 
ELCTYGCDST KLGKKRFYQK LADILMEVTK RTRELVECVK MANRQTLSQD LSSFFNYRSV 


Q 

References and documentation are available.

Number of amino acids: 661

Molecular weight: 74006.57

Theoretical pI: 9.02

Amino acid composition: 
Ala (A)  29	  4.4%
Arg (R)  35	  5.3%
Asn (N)  43	  6.5%
Asp (D)  40	  6.1%
Cys (C)   7	  1.1%
Gln (Q)  24	  3.6%
Glu (E)  40	  6.1%
Gly (G)  25	  3.8%
His (H)   8	  1.2%
Ile (I)  53	  8.0%
Leu (L)  66	 10.0%
Lys (K)  56	  8.5%
Met (M)  13	  2.0%
Phe (F)  24	  3.6%
Pro (P)  27	  4.1%
Ser (S)  77	 11.6%
Thr (T)  42	  6.4%
Trp (W)   1	  0.2%
Tyr (Y)  12	  1.8%
Val (V)  39	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      3247
Hydrogen    H	      5314
Nitrogen    N	       906
Oxygen      O	      1020
Sulfur      S	        20

Formula: C3247H5314N906O1020S20
Total number of atoms: 10507

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23755
Abs 0.1% (=1 g/l)   0.321, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.316, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.15
This classifies the protein as unstable.



Aliphatic index: 91.71

Grand average of hydropathicity (GRAVY): -0.396