ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MISSIKPVLP SLTAIVGIGI YFAIWWALNG VFNNYNKKVL NAFPYLWLTL TLSLACGSLM 

        70         80         90        100        110        120 
MLVSWVALAH TIGHVEAIVR CALAAVMELN FNMIGFMGAM ISNLAFVFRN IFSKKGMKGK 

       130        140        150        160        170        180 
SVSVMNYYAC LSMMSLLIVT PFANSVEGPQ MWADGWQNDV SKSDQTLSSK WVVAHSVFYH 

       190        200        210        220        230        240 
LYNQVSYIPR CLNHHLPNPL KHVNALGAAI AILGTFIYSQ IKNRVKKNHI LLVLCLGMLE 


PLVITL 

References and documentation are available.

Number of amino acids: 246

Molecular weight: 27341.64

Theoretical pI: 9.49

Amino acid composition: 
Ala (A)  21	  8.5%
Arg (R)   4	  1.6%
Asn (N)  18	  7.3%
Asp (D)   3	  1.2%
Cys (C)   5	  2.0%
Gln (Q)   5	  2.0%
Glu (E)   4	  1.6%
Gly (G)  14	  5.7%
His (H)   8	  3.3%
Ile (I)  19	  7.7%
Leu (L)  32	 13.0%
Lys (K)  13	  5.3%
Met (M)  13	  5.3%
Phe (F)  11	  4.5%
Pro (P)   9	  3.7%
Ser (S)  20	  8.1%
Thr (T)   8	  3.3%
Trp (W)   7	  2.8%
Tyr (Y)   9	  3.7%
Val (V)  23	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon      C	      1265
Hydrogen    H	      1979
Nitrogen    N	       317
Oxygen      O	       321
Sulfur      S	        18

Formula: C1265H1979N317O321S18
Total number of atoms: 3900

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52160
Abs 0.1% (=1 g/l)   1.908, assuming all pairs of Cys residues form cystines


Ext. coefficient    51910
Abs 0.1% (=1 g/l)   1.899, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.49
This classifies the protein as stable.



Aliphatic index: 116.50

Grand average of hydropathicity (GRAVY): 0.612