ProtParam
User-provided sequence:
10 20 30 40 50 60
MITGKELRII SLLTLDTVFF LLEITIGYMS HSLALIADSF HMLNDIISLL VALWAVDVAK
70 80 90 100 110 120
NRGPDAKYTY GWKRAEILGA LINAVFLIAL CFSIMIEALQ RLIEPQEIQN PRLVLYVGVA
130 140 150 160 170 180
GLISNVVGLF LFHDHGSDSL HSHSHGSVES GNNDLDIESN ATHSHSHASL PNDNLAIDED
190 200 210 220 230 240
AISSPGPSGQ IGEVLPQSVV NRLSNESQPL LNHDDHDHSH ESKKPGHRSL NMHGVFLHVL
250 260 270 280 290 300
GDALGNIGVI AAALFIWKTE YSWRYYSDPI VSLIITIIIF SSALPLSRRA SRILLQATPS
310 320 330 340 350 360
TISADQIQRE ILAVPGVIAV HDFHVWNLTE SIYIASIHVQ IDCAPDKFMS SAKLIRKIFH
370 380 390 400 410 420
QHGIHSATVQ PEFVSGDVNE DIRRRFSIIA GGSPSSSQEA FDSHGNTEHG RKKRSPTAYG
430 440
ATTASSNCIV DDAVNCNTSN CL
References and
documentation are available.
Number of amino acids: 442
Molecular weight: 48344.87
Theoretical pI: 5.88
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 2155
Hydrogen H 3386
Nitrogen N 598
Oxygen O 645
Sulfur S 11
Formula: C
2155H
3386N
598O
645S
11
Total number of atoms: 6795
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41160
Abs 0.1% (=1 g/l) 0.851, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.846, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.40
This classifies the protein as unstable.
Aliphatic index: 105.93
Grand average of hydropathicity (GRAVY): 0.068