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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITPRFSITQ DEEFIFLKIF ISNIRFSAVG LEIIIQENMI IFHLSPYYLR LRFPHELIDD

70 80 90 100 110 120
ERSTAQYDSK DECINVKVAK LNKNEYFEDL DLPTKLLARQ GDLAGADALT ENTDAKKTQK

130 140 150 160 170 180
PLIQEVETDG VSNNIKDDVK TIGQMGEGFN WEIEQKMDSS TNNGILKTKY GFDNLYDTVI

190 200 210 220 230 240
SVSTSNGNDI NELDDPEHTD ANDRVIERLR KENLKFDPEY YVSEYMTHKY GNEEDLEING

250 260 270 280 290 300
IKELLKFTPS IVKQYLQWYK DSTNPNLVMP IEFTDEEQKQ MQDNLPKKSY LVEDIKPLYV

310 320 330 340 350 360
TILSVLFSYV FEQIENEGTH TTESAWTMGK LCPQISFLDQ QLKQVNELQD GMKEISKVNK

370 380 390 400 410 420
DSSLIKIAII TGIRRALSYP LHRNYDLAMK AWTFVYYILR GGKRLVIRAL LDIHETFRFH

430 440 450 460 470 480
DVYYVYDKVL LDDLTAWFIS QGSENVIRSL ALEMRKEQES LSKQDIEFEC IASFNEQTGE

490 500
PEWETLNIRE MEILAESEYR EQQQNPQ


References and documentation are available.

Number of amino acids: 507 Molecular weight: 59150.79 Theoretical pI: 4.72
Amino acid composition: 
Ala (A) 19 3.7% Arg (R) 20 3.9% Asn (N) 30 5.9% Asp (D) 37 7.3% Cys (C) 3 0.6% Gln (Q) 27 5.3% Glu (E) 51 10.1% Gly (G) 20 3.9% His (H) 8 1.6% Ile (I) 44 8.7% Leu (L) 49 9.7% Lys (K) 36 7.1% Met (M) 12 2.4% Phe (F) 22 4.3% Pro (P) 16 3.2% Ser (S) 31 6.1% Thr (T) 29 5.7% Trp (W) 6 1.2% Tyr (Y) 23 4.5% Val (V) 24 4.7% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 88 Total number of positively charged residues (Arg + Lys): 56 Atomic composition: Carbon C 2646 Hydrogen H 4120 Nitrogen N 682 Oxygen O 824 Sulfur S 15 Formula: C2646H4120N682O824S15 Total number of atoms: 8287 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 67395 Abs 0.1% (=1 g/l) 1.139, assuming all pairs of Cys residues form cystines Ext. coefficient 67270 Abs 0.1% (=1 g/l) 1.137, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.40 This classifies the protein as unstable. Aliphatic index: 89.01 Grand average of hydropathicity (GRAVY): -0.525