ProtParam
User-provided sequence:
10 20 30 40 50 60
MITPRFSITQ DEEFIFLKIF ISNIRFSAVG LEIIIQENMI IFHLSPYYLR LRFPHELIDD
70 80 90 100 110 120
ERSTAQYDSK DECINVKVAK LNKNEYFEDL DLPTKLLARQ GDLAGADALT ENTDAKKTQK
130 140 150 160 170 180
PLIQEVETDG VSNNIKDDVK TIGQMGEGFN WEIEQKMDSS TNNGILKTKY GFDNLYDTVI
190 200 210 220 230 240
SVSTSNGNDI NELDDPEHTD ANDRVIERLR KENLKFDPEY YVSEYMTHKY GNEEDLEING
250 260 270 280 290 300
IKELLKFTPS IVKQYLQWYK DSTNPNLVMP IEFTDEEQKQ MQDNLPKKSY LVEDIKPLYV
310 320 330 340 350 360
TILSVLFSYV FEQIENEGTH TTESAWTMGK LCPQISFLDQ QLKQVNELQD GMKEISKVNK
370 380 390 400 410 420
DSSLIKIAII TGIRRALSYP LHRNYDLAMK AWTFVYYILR GGKRLVIRAL LDIHETFRFH
430 440 450 460 470 480
DVYYVYDKVL LDDLTAWFIS QGSENVIRSL ALEMRKEQES LSKQDIEFEC IASFNEQTGE
490 500
PEWETLNIRE MEILAESEYR EQQQNPQ
References and
documentation are available.
Number of amino acids: 507
Molecular weight: 59150.79
Theoretical pI: 4.72
Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2646
Hydrogen H 4120
Nitrogen N 682
Oxygen O 824
Sulfur S 15
Formula: C
2646H
4120N
682O
824S
15
Total number of atoms: 8287
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67395
Abs 0.1% (=1 g/l) 1.139, assuming all pairs of Cys residues form cystines
Ext. coefficient 67270
Abs 0.1% (=1 g/l) 1.137, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.40
This classifies the protein as unstable.
Aliphatic index: 89.01
Grand average of hydropathicity (GRAVY): -0.525