ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITQNSIPEV KEDFIGYALH ERRIRLPQFQ DLGPADLVTL TKYLPTSSNT NAINSTSRNG 

        70         80         90        100        110        120 
AAIIQSPAAV VADDSAASMA TNGDASDTAV TTNYTNASIY SSSRNANDGA PMVAELHPLD 

       130        140        150        160        170        180 
KLKDEVGTFF YSMGVDTSGP TSIAIFLKEI SEVISEKPQV WFGRKKTFNV ARISFSTWNA 

       190        200        210        220        230        240 
FRRCDINVVV HIPGSIQNFI VDCNGESQNI EMCADYDLIW AETFVSGVVR SIMLMKENAE 

       250        260        270        280        290        300 
EGELQNLVET LILNPFTAGQ IDDVPEMFID LFPIVYHKGP LLGAPYYITN VTNTNNYLVE 

       310        320        330        340        350        360 
TLVEIVKLTR NVSRAEIMLK NLATDNPEAI IILIKIFLVC DQELDAIKLT YDMLSQDKII 

       370        380        390        400        410        420 
NNTNNRMDYK SELLCLQAQF LIDKRQDYSL AQNIAQEAVN CSPSEFRPWY LLSKVYVKLN 

       430        440        450        460        470        480 
DIENALLILN SCPMSPLKEK YVLKRVAPLP SNNSLHLPLP IDVVLDEVTS LNPQDVQNEH 

       490        500        510        520        530        540 
RSADPMLVNL AASNLKSTFQ LAYRLLTEIV QITGWENLLK YRSNIFVMEE EYQKSSSSLP 

       550        560        570        580        590        600 
KDVNKQEEQP LRAKRLCERW LDNLFMLLYE DLKMYTLWQT EQLYMDAQNN NHNKLTFEWE 

       610        620        630        640        650        660 
LFGLCARRLG HFPEAAKAFQ NGLSQRFSSR CARKLLEYCI NERQRVKNFI NSPNSHDMVP 

       670        680        690        700        710        720 
EIVSSRIREL DNSIIDLCVK ICCWNHRWYT EFSISLLDCL SVVIQDMSLT KVSNEISSRY 

       730        740 
PETVLNLVQE NLLNFFTTCT IGCYDA 

References and documentation are available.

Number of amino acids: 746

Molecular weight: 84829.92

Theoretical pI: 5.04

Amino acid composition: 
Ala (A)  47	  6.3%
Arg (R)  33	  4.4%
Asn (N)  60	  8.0%
Asp (D)  41	  5.5%
Cys (C)  17	  2.3%
Gln (Q)  31	  4.2%
Glu (E)  50	  6.7%
Gly (G)  21	  2.8%
His (H)  10	  1.3%
Ile (I)  54	  7.2%
Leu (L)  83	 11.1%
Lys (K)  35	  4.7%
Met (M)  19	  2.5%
Phe (F)  29	  3.9%
Pro (P)  32	  4.3%
Ser (S)  59	  7.9%
Thr (T)  40	  5.4%
Trp (W)  10	  1.3%
Tyr (Y)  26	  3.5%
Val (V)  49	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      3775
Hydrogen    H	      5947
Nitrogen    N	      1001
Oxygen      O	      1145
Sulfur      S	        36

Formula: C3775H5947N1001O1145S36
Total number of atoms: 11904

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94740
Abs 0.1% (=1 g/l)   1.117, assuming all pairs of Cys residues form cystines


Ext. coefficient    93740
Abs 0.1% (=1 g/l)   1.105, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.98
This classifies the protein as unstable.



Aliphatic index: 96.97

Grand average of hydropathicity (GRAVY): -0.165