ProtParam
User-provided sequence:
10 20 30 40 50 60
MITQPAKCPL LIRISAFRSG SSFLLYVHCK SINKSWQPKP GNPLRNARLN YINIKDMWRE
70 80 90 100 110 120
ASLPSHFAFH DLKWFFHNRR APTRNMAVGG NNWSMWLRMS RVHLRQITKS LDRTLISLSH
130 140 150 160 170 180
GNFSHQYNRN IFVTWWKSLF EASTAFRRAS GLTVSPLTRR GIARFDHFRP VPNVSKFASF
190 200 210 220 230 240
PRVPKGAPRG LFTNWNMTTS KRLLGQRAYS TSSIKFTQEA VNNMTISLRC FFNSLGGLNQ
250 260 270 280 290 300
CSHSNSCKAY QNASNVTSKQ DHVQPVALKK LSQKDINFIR NLELFKIMKT QNEVVDETSA
310 320 330 340 350 360
YYMEKPGSYI EFTISEFNVN GTFSAPLSFL DPSLLADLDE MIRNYKYELK SIYSSVDMIL
370 380 390 400 410 420
QNYGSLPITF HRNKIRIHFP NSTVVETEKL IAGLNIATGV IYADTSPDIS LEGTNLNALV
430 440 450
NVDNSGSVWS FVKEPSFPSR SAFSPILSDA SYDTYELV
References and
documentation are available.
Number of amino acids: 458
Molecular weight: 52183.63
Theoretical pI: 9.84
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2348
Hydrogen H 3636
Nitrogen N 652
Oxygen O 667
Sulfur S 16
Formula: C
2348H
3636N
652O
667S
16
Total number of atoms: 7319
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72100
Abs 0.1% (=1 g/l) 1.382, assuming all pairs of Cys residues form cystines
Ext. coefficient 71850
Abs 0.1% (=1 g/l) 1.377, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.98
This classifies the protein as stable.
Aliphatic index: 79.85
Grand average of hydropathicity (GRAVY): -0.300