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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITQPAKCPL LIRISAFRSG SSFLLYVHCK SINKSWQPKP GNPLRNARLN YINIKDMWRE

70 80 90 100 110 120
ASLPSHFAFH DLKWFFHNRR APTRNMAVGG NNWSMWLRMS RVHLRQITKS LDRTLISLSH

130 140 150 160 170 180
GNFSHQYNRN IFVTWWKSLF EASTAFRRAS GLTVSPLTRR GIARFDHFRP VPNVSKFASF

190 200 210 220 230 240
PRVPKGAPRG LFTNWNMTTS KRLLGQRAYS TSSIKFTQEA VNNMTISLRC FFNSLGGLNQ

250 260 270 280 290 300
CSHSNSCKAY QNASNVTSKQ DHVQPVALKK LSQKDINFIR NLELFKIMKT QNEVVDETSA

310 320 330 340 350 360
YYMEKPGSYI EFTISEFNVN GTFSAPLSFL DPSLLADLDE MIRNYKYELK SIYSSVDMIL

370 380 390 400 410 420
QNYGSLPITF HRNKIRIHFP NSTVVETEKL IAGLNIATGV IYADTSPDIS LEGTNLNALV

430 440 450
NVDNSGSVWS FVKEPSFPSR SAFSPILSDA SYDTYELV


References and documentation are available.

Number of amino acids: 458 Molecular weight: 52183.63 Theoretical pI: 9.84
Amino acid composition: 
Ala (A) 27 5.9% Arg (R) 29 6.3% Asn (N) 36 7.9% Asp (D) 16 3.5% Cys (C) 5 1.1% Gln (Q) 13 2.8% Glu (E) 16 3.5% Gly (G) 19 4.1% His (H) 12 2.6% Ile (I) 28 6.1% Leu (L) 41 9.0% Lys (K) 25 5.5% Met (M) 11 2.4% Phe (F) 29 6.3% Pro (P) 23 5.0% Ser (S) 54 11.8% Thr (T) 26 5.7% Trp (W) 9 2.0% Tyr (Y) 15 3.3% Val (V) 24 5.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 32 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2348 Hydrogen H 3636 Nitrogen N 652 Oxygen O 667 Sulfur S 16 Formula: C2348H3636N652O667S16 Total number of atoms: 7319 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 72100 Abs 0.1% (=1 g/l) 1.382, assuming all pairs of Cys residues form cystines Ext. coefficient 71850 Abs 0.1% (=1 g/l) 1.377, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.98 This classifies the protein as stable. Aliphatic index: 79.85 Grand average of hydropathicity (GRAVY): -0.300