ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITQTELDNC LQWAQNNGAF IDPKISFRIT EDAGVSAFVN EKFSPKPDQA LIRVPETLLI 

        70         80         90        100        110        120 
TSQQALSEFS QAANERSLLN SVTQLYLSKL KFGTDAVHLK SFYKPYLDVL PLHLPQPYFW 

       130        140        150        160        170        180 
STDEVMNLHG TDVYLTMRDT LNKLVKEWRM LFQALSIEHS SQDKQFLSLF QENKDSAVVP 

       190        200        210        220        230        240 
LEQFCAHING CKLEDSEWNS FVAYLWSYCI FNSRAFPRVI LGRAGTDRTN LNEGFLYPIV 

       250        260        270        280        290        300 
DLLNHKNDVP VRWEMNEQNE LCFMSQTTTF SAQDELFNNY GNISNEKCLL NYGFWDSSNK 

       310        320        330        340        350        360 
FDFSRLTLKL PSTLVSGLPV DFNKSGNFVT DDGETTILQF SLKISEPLPP VLLALFAYLS 

       370        380        390        400        410        420 
KLKSEETPTV RSVLEGIDQL TSVVSQRLLF YKNFKIKTSS TQKLRPHVIK LIKLYYQDNK 

       430        440        450        460        470        480 
KILNATTEKL SVLQKKIYSN NKEFSLSFKT IFKNDKIFAN SLLLVFGAIN YEDLITKDCL 

       490        500        510        520        530        540 
NDALLLWIVK LINDKSNNQG GFIKQTFKEV SDSIVIEKED VMEFLPFYKK YFPNLSERIP 

       550        560        570        580 
EIYSVGDWGI RQFIVADTAI DRLVWIRKSN KEPIFLMKKA YDLQI 

References and documentation are available.

Number of amino acids: 585

Molecular weight: 67452.45

Theoretical pI: 6.25

Amino acid composition: 
Ala (A)  25	  4.3%
Arg (R)  18	  3.1%
Asn (N)  40	  6.8%
Asp (D)  34	  5.8%
Cys (C)   7	  1.2%
Gln (Q)  27	  4.6%
Glu (E)  34	  5.8%
Gly (G)  19	  3.2%
His (H)   7	  1.2%
Ile (I)  37	  6.3%
Leu (L)  74	 12.6%
Lys (K)  47	  8.0%
Met (M)   8	  1.4%
Phe (F)  40	  6.8%
Pro (P)  23	  3.9%
Ser (S)  49	  8.4%
Thr (T)  32	  5.5%
Trp (W)  10	  1.7%
Tyr (Y)  20	  3.4%
Val (V)  34	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      3083
Hydrogen    H	      4781
Nitrogen    N	       777
Oxygen      O	       890
Sulfur      S	        15

Formula: C3083H4781N777O890S15
Total number of atoms: 9546

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    85175
Abs 0.1% (=1 g/l)   1.263, assuming all pairs of Cys residues form cystines


Ext. coefficient    84800
Abs 0.1% (=1 g/l)   1.257, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.50
This classifies the protein as stable.



Aliphatic index: 95.13

Grand average of hydropathicity (GRAVY): -0.205