ProtParam
User-provided sequence:
10 20 30 40 50 60
MITQTELDNC LQWAQNNGAF IDPKISFRIT EDAGVSAFVN EKFSPKPDQA LIRVPETLLI
70 80 90 100 110 120
TSQQALSEFS QAANERSLLN SVTQLYLSKL KFGTDAVHLK SFYKPYLDVL PLHLPQPYFW
130 140 150 160 170 180
STDEVMNLHG TDVYLTMRDT LNKLVKEWRM LFQALSIEHS SQDKQFLSLF QENKDSAVVP
190 200 210 220 230 240
LEQFCAHING CKLEDSEWNS FVAYLWSYCI FNSRAFPRVI LGRAGTDRTN LNEGFLYPIV
250 260 270 280 290 300
DLLNHKNDVP VRWEMNEQNE LCFMSQTTTF SAQDELFNNY GNISNEKCLL NYGFWDSSNK
310 320 330 340 350 360
FDFSRLTLKL PSTLVSGLPV DFNKSGNFVT DDGETTILQF SLKISEPLPP VLLALFAYLS
370 380 390 400 410 420
KLKSEETPTV RSVLEGIDQL TSVVSQRLLF YKNFKIKTSS TQKLRPHVIK LIKLYYQDNK
430 440 450 460 470 480
KILNATTEKL SVLQKKIYSN NKEFSLSFKT IFKNDKIFAN SLLLVFGAIN YEDLITKDCL
490 500 510 520 530 540
NDALLLWIVK LINDKSNNQG GFIKQTFKEV SDSIVIEKED VMEFLPFYKK YFPNLSERIP
550 560 570 580
EIYSVGDWGI RQFIVADTAI DRLVWIRKSN KEPIFLMKKA YDLQI
References and
documentation are available.
Number of amino acids: 585
Molecular weight: 67452.45
Theoretical pI: 6.25
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 3083
Hydrogen H 4781
Nitrogen N 777
Oxygen O 890
Sulfur S 15
Formula: C
3083H
4781N
777O
890S
15
Total number of atoms: 9546
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 85175
Abs 0.1% (=1 g/l) 1.263, assuming all pairs of Cys residues form cystines
Ext. coefficient 84800
Abs 0.1% (=1 g/l) 1.257, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.50
This classifies the protein as stable.
Aliphatic index: 95.13
Grand average of hydropathicity (GRAVY): -0.205