ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITRLSGACL RRSGAKRNWP REHLVHRSLL ASFSTTQRVL KCSVGPFRTS NVVFKSKEPK 

        70         80         90        100        110        120 
DNKPLDNKND PKKTHNEDES HTNESLPSTD KKKKKDNDDF KQNLKSSSNK TEEKYSATQA 

       130        140        150        160        170        180 
SKSKNDEFEL GGEENEDEMP LNGEFNKNVP AKYSVPDVYP QLLALPIARR PLFPGFYKAI 

       190        200        210        220        230        240 
VTKNPSVSEA IKELIKKRQP YIGAFLLKDE NTDTDVITNI DQVYPVGVFA QITSIFPAKS 

       250        260        270        280        290        300 
GSEPALTAVL YPHRRIRITE LIPPKEDADS AASSDAAELE TDKSSNLSSN GEVKSDLKQD 

       310        320        330        340        350        360 
NGKEEPEKEV ESTPSILQNF KVSLVNVENV PNEPFKRQDP VIKAVTSEIM NVFKDIANVS 

       370        380        390        400        410        420 
PLFREQIANF SISQTSGNVF DEPAKLADFA AAVSAADHRE LQEVLEATNI GDRLQKALYV 

       430        440        450        460        470        480 
LKKELLNAQL QHKINKEIEQ KITQRHKEYL LTEQLKQIKR ELGQELDSKE ALVTEFKKRT 

       490        500        510        520        530        540 
ESLSMPDHVK KVFNDELSKF QHLEPMAAEF NITRNYLDWI TQLPWGKRSV ENFDLDHAKE 

       550        560        570        580        590        600 
VLDRDHYGLK DVKDRVLELV AVGKLRGTMQ GKIMCLVGPP GVGKTSVGKS IASALNREFF 

       610        620        630        640        650        660 
RFSVGGLTDV AEIKGHRRTY IGAMPGKIVQ ALKKVQTENP LILIDEIDKV GKSHQGDPAS 

       670        680        690        700        710        720 
ALLELLDSEQ NSAFLDYYMD IPLDVSSVLF VCTANTIDTI PPPLLDRMEV IELSGYVSAE 

       730        740        750        760        770        780 
KVNIAKGYLI PQAKAACGLK DANVNISDDA IKGLISYYAH ESGVRNLKKS IEKIFRKTSF 

       790        800        810        820        830        840 
SIVKEIDDEL NSKEKSTGKS GKKTSPQSSE DAANKEASSV PLKVPDKVNI EIEEKDLTKY 

       850        860        870        880        890        900 
LGPPIYTSQR LYDTTPPGVV MGLGWTPMGG VSMYVETIVK NILSSNSTPS LERTGQLGDV 

       910        920        930        940        950        960 
MKESSEISYS FSKSFLSKHF PNNKFFEHAR LHMHCPEGSI SKDGPSAGIT MATSLLSLAL 

       970        980        990       1000       1010       1020 
DTPVPATTAM TGELTLTGKI LRIGGLREKT VAAKLSGMKE ILFPKSNLAD WEQLPDYVKE 

      1030       1040       1050       1060 
GLTGVPVAWY DDVFKRVFSN IDAEKCNNLW PNLIKSSSKQ HQISPSH 

References and documentation are available.

Number of amino acids: 1067

Molecular weight: 118641.99

Theoretical pI: 6.76

Amino acid composition: 
Ala (A)  69	  6.5%
Arg (R)  39	  3.7%
Asn (N)  56	  5.2%
Asp (D)  64	  6.0%
Cys (C)   7	  0.7%
Gln (Q)  35	  3.3%
Glu (E)  83	  7.8%
Gly (G)  56	  5.2%
His (H)  21	  2.0%
Ile (I)  61	  5.7%
Leu (L)  99	  9.3%
Lys (K) 105	  9.8%
Met (M)  18	  1.7%
Phe (F)  38	  3.6%
Pro (P)  60	  5.6%
Ser (S)  97	  9.1%
Thr (T)  56	  5.2%
Trp (W)   7	  0.7%
Tyr (Y)  25	  2.3%
Val (V)  71	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 147
Total number of positively charged residues (Arg + Lys): 144

Atomic composition:

Carbon      C	      5264
Hydrogen    H	      8437
Nitrogen    N	      1429
Oxygen      O	      1631
Sulfur      S	        25

Formula: C5264H8437N1429O1631S25
Total number of atoms: 16786

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76125
Abs 0.1% (=1 g/l)   0.642, assuming all pairs of Cys residues form cystines


Ext. coefficient    75750
Abs 0.1% (=1 g/l)   0.638, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.42
This classifies the protein as unstable.



Aliphatic index: 84.25

Grand average of hydropathicity (GRAVY): -0.495