ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MITTVQEISK WRNLCFIRMQ SRKWYPVLKK TPLVADGRKI IKHADKVPHP EEIIHPFYQP 

        70         80         90        100        110        120 
TAIEQFTACA TEYNPSLLDG KKIAPSLIKH PVSLKTILVD SKLKFDDIRG VNKWLMEFVA 

       130        140        150        160        170        180 
RRQHQRNIVL TPASKSVRSF HVLHLSSTDI AKLRGLENIL SEIENTNDLQ SRVESVNNEL 

       190        200        210        220        230        240 
QNIFDRDSKQ TRLFCENILA YLIKNYGNST EKLILLINVT EMQLYSRLDQ MKAMNIILYN 

       250        260        270        280        290        300 
ILCKVEANEN PPYSPTLVTA LENLLAAINN RFFPGRCENS LHPIVIEQLL SYFIKTGNLN 

       310        320        330        340        350        360 
ESKNFLGHLI KKGILPEATI INRYLEAIDV HFDKSTKIFD IRSKFAFIAD LAPIIENYGT 

       370        380        390        400        410        420 
IDLFKFLIPM CRHFDELCSL LNIIRKSNNA KRAVDSTLPI FIKKVLTFTK DPMINSGNLS 

       430        440        450        460        470        480 
TVFNIVSPIY GQNVPSEFVE KFILSFALQG NYTMMAHMID TYKIKLSHKY QLQIIRALKN 

       490        500        510 
SERNHALKNT GAVGYNKEFK KYFIEKYLNC TEREALRP 

References and documentation are available.

Number of amino acids: 518

Molecular weight: 59747.68

Theoretical pI: 9.35

Amino acid composition: 
Ala (A)  28	  5.4%
Arg (R)  25	  4.8%
Asn (N)  40	  7.7%
Asp (D)  20	  3.9%
Cys (C)   8	  1.5%
Gln (Q)  16	  3.1%
Glu (E)  31	  6.0%
Gly (G)  15	  2.9%
His (H)  14	  2.7%
Ile (I)  53	 10.2%
Leu (L)  57	 11.0%
Lys (K)  44	  8.5%
Met (M)  11	  2.1%
Phe (F)  27	  5.2%
Pro (P)  23	  4.4%
Ser (S)  33	  6.4%
Thr (T)  27	  5.2%
Trp (W)   3	  0.6%
Tyr (Y)  18	  3.5%
Val (V)  25	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2711
Hydrogen    H	      4322
Nitrogen    N	       724
Oxygen      O	       755
Sulfur      S	        19

Formula: C2711H4322N724O755S19
Total number of atoms: 8531

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43820
Abs 0.1% (=1 g/l)   0.733, assuming all pairs of Cys residues form cystines


Ext. coefficient    43320
Abs 0.1% (=1 g/l)   0.725, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.68
This classifies the protein as unstable.



Aliphatic index: 102.22

Grand average of hydropathicity (GRAVY): -0.175