ProtParam
User-provided sequence:
10 20 30 40 50 60
MITVRQFTFT IFKFQFMATS NVSSRVNELL EAVKKEFEDI CQKTKTVEAQ KDDFEYKAMI
70 80 90 100 110 120
SAQINEMALM KQTVMDLEMQ QSKVKDRYEE EITSLKAQLE ARRKEIASGV VPQSSKTKHG
130 140 150 160 170 180
RNSVSFGKYG NAGPFNSDNS SKPLILNNGS SGGTPKNLRS PAIDSDGTVL APIQTSNVDL
190 200 210 220 230 240
GSQYYSSPHV RPAVGATMAG SAMRTFPSNL PLGHPPPPSD SANSSVTPIA APLVVNGKVS
250 260 270 280 290 300
GNPPYPAEII PTSNVPNREE KDWTVTSNVP NKEPPISVQL LHTLEHTSVI CYVRFSADGK
310 320 330 340 350 360
FLATGCNRAA MVFNVETGKL ITLLQEESSK REGDLYVRSV AFSPDGKYLA TGVEDQQIRI
370 380 390 400 410 420
WDIAQKRVYR LLTGHEQEIY SLDFSKDGKT LVSGSGDRTV CLWDVEAGEQ KLILHTDDGV
430 440 450 460 470 480
TTVMFSPDGQ FIAAGSLDKV IRIWTSSGTL VEQLHGHEES VYSVAFSPDG KYLVSGSLDN
490 500 510 520 530 540
TIKLWELQCV SNVAPSMYKE GGICKQTFTG HKDFILSVTV SPDGKWIISG SKDRTIQFWS
550 560 570 580
PDSPHSQLTL QGHNNSVISV AVSPNGHCFA TGSGDLRARI WSYEDL
References and
documentation are available.
Number of amino acids: 586
Molecular weight: 64192.38
Theoretical pI: 6.05
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2834
Hydrogen H 4460
Nitrogen N 774
Oxygen O 888
Sulfur S 19
Formula: C
2834H
4460N
774O
888S
19
Total number of atoms: 8975
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66725
Abs 0.1% (=1 g/l) 1.039, assuming all pairs of Cys residues form cystines
Ext. coefficient 66350
Abs 0.1% (=1 g/l) 1.034, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.23
This classifies the protein as stable.
Aliphatic index: 78.48
Grand average of hydropathicity (GRAVY): -0.339