ProtParam
User-provided sequence:
10 20 30 40 50 60
MIVPTYGDVL DASNRIKEYV NKTPVLTSRM LNDRLGAQIY FKGENFQRVG AFKFRGAMNA
70 80 90 100 110 120
VSKLSDEKRS KGVIAFSSGN HAQAIALSAK LLNVPATIVM PEDAPALKVA ATAGYGAHII
130 140 150 160 170 180
RYNRYTEDRE QIGRQLAAEH GFALIPPYDH PDVIAGQGTS AKELLEEVGQ LDALFVPLGG
190 200 210 220 230 240
GGLLSGSALA ARSLSPGCKI FGVEPEAGND GQQSFRSGSI VHINTPKTIA DGAQTQHLGE
250 260 270 280 290 300
YTFAIIRENV DDILTVSDQE LVKCMHFLAE RMKVVVEPTA CLGFAGALLK KEELVGKKVG
310 320
IILSGGNVDM KRYATLISGK EDGPTI
References and
documentation are available.
Number of amino acids: 326
Molecular weight: 34899.00
Theoretical pI: 6.67
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1549
Hydrogen H 2489
Nitrogen N 429
Oxygen O 466
Sulfur S 10
Formula: C
1549H
2489N
429O
466S
10
Total number of atoms: 4943
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 13535
Abs 0.1% (=1 g/l) 0.388, assuming all pairs of Cys residues form cystines
Ext. coefficient 13410
Abs 0.1% (=1 g/l) 0.384, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.65
This classifies the protein as stable.
Aliphatic index: 94.29
Grand average of hydropathicity (GRAVY): -0.062