ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF 

        70 
LRYNPLEKRD 

References and documentation are available.

Number of amino acids: 70

Molecular weight: 8277.71

Theoretical pI: 7.65

Amino acid composition: 
Ala (A)   1	  1.4%
Arg (R)   7	 10.0%
Asn (N)   2	  2.9%
Asp (D)   5	  7.1%
Cys (C)   4	  5.7%
Gln (Q)   1	  1.4%
Glu (E)   6	  8.6%
Gly (G)   4	  5.7%
His (H)   1	  1.4%
Ile (I)   3	  4.3%
Leu (L)  11	 15.7%
Lys (K)   5	  7.1%
Met (M)   2	  2.9%
Phe (F)   2	  2.9%
Pro (P)   2	  2.9%
Ser (S)   3	  4.3%
Thr (T)   2	  2.9%
Trp (W)   1	  1.4%
Tyr (Y)   3	  4.3%
Val (V)   5	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 12

Atomic composition:

Carbon      C	       366
Hydrogen    H	       592
Nitrogen    N	       102
Oxygen      O	       104
Sulfur      S	         6

Formula: C366H592N102O104S6
Total number of atoms: 1170

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10220
Abs 0.1% (=1 g/l)   1.235, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   1.204, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.75
This classifies the protein as unstable.



Aliphatic index: 100.14

Grand average of hydropathicity (GRAVY): -0.273