ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKAAVLAVAL VFLTGCQAWE FWQQDEPQSQ WDRVKDFATV YVDAVKDSGR DYVSQFESST 

        70         80         90        100        110        120 
LGKQLNLNLL DNWDTLGSTV GRLQEQLGPV TQEFWANLEK ETDWLRNEMN KDLENVKQKM 

       130        140        150        160        170        180 
QPHLDEFQEK WNEEVEAYRQ KLEPLGTELH KNAKEMQRHL KVVAEEFRDR MRVNADALRA 

       190        200        210        220        230        240 
KFGLYSDQMR ENLAQRLTEI KNHPTLIEYH TKASDHLKTL GEKAKPALDD LGQGLMPVLE 

       250 
AWKAKIMSMI DEAKKKLNA 

References and documentation are available.

Number of amino acids: 259

Molecular weight: 30062.13

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  22	  8.5%
Arg (R)  12	  4.6%
Asn (N)  13	  5.0%
Asp (D)  18	  6.9%
Cys (C)   1	  0.4%
Gln (Q)  18	  6.9%
Glu (E)  25	  9.7%
Gly (G)  11	  4.2%
His (H)   6	  2.3%
Ile (I)   4	  1.5%
Leu (L)  31	 12.0%
Lys (K)  24	  9.3%
Met (M)   9	  3.5%
Phe (F)   8	  3.1%
Pro (P)   7	  2.7%
Ser (S)   9	  3.5%
Thr (T)  12	  4.6%
Trp (W)   8	  3.1%
Tyr (Y)   5	  1.9%
Val (V)  16	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1332
Hydrogen    H	      2096
Nitrogen    N	       370
Oxygen      O	       403
Sulfur      S	        10

Formula: C1332H2096N370O403S10
Total number of atoms: 4211

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51450
Abs 0.1% (=1 g/l)   1.711, assuming all pairs of Cys residues form cystines


Ext. coefficient    51450
Abs 0.1% (=1 g/l)   1.711, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.36
This classifies the protein as stable.



Aliphatic index: 79.11

Grand average of hydropathicity (GRAVY): -0.719