ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKAIDKMTDN PPQEGLSGRK IIYDEDGKPC RSCNTLLDFQ YVTGKISNGL KNLSSNGKLA 

        70         80         90        100        110        120 
GTGALTGEAS ELMPGSRTYR KVDPPDVEQL GRSSWTLLHS VAASYPAQPT DQQKGEMKQF 

       130        140        150        160        170        180 
LNIFSHIYPC NWCAKDFEKY IRENAPQVES REELGRWMCE AHNKVNKKLR KPKFDCNFWE 


KRWKDGWDE 

References and documentation are available.

Number of amino acids: 189

Molecular weight: 21639.48

Theoretical pI: 8.46

Amino acid composition: 
Ala (A)  10	  5.3%
Arg (R)  10	  5.3%
Asn (N)  11	  5.8%
Asp (D)  12	  6.3%
Cys (C)   6	  3.2%
Gln (Q)   8	  4.2%
Glu (E)  14	  7.4%
Gly (G)  14	  7.4%
His (H)   3	  1.6%
Ile (I)   7	  3.7%
Leu (L)  14	  7.4%
Lys (K)  19	 10.1%
Met (M)   5	  2.6%
Phe (F)   6	  3.2%
Pro (P)  11	  5.8%
Ser (S)  13	  6.9%
Thr (T)   8	  4.2%
Trp (W)   6	  3.2%
Tyr (Y)   6	  3.2%
Val (V)   6	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	       951
Hydrogen    H	      1477
Nitrogen    N	       269
Oxygen      O	       288
Sulfur      S	        11

Formula: C951H1477N269O288S11
Total number of atoms: 2996

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42315
Abs 0.1% (=1 g/l)   1.955, assuming all pairs of Cys residues form cystines


Ext. coefficient    41940
Abs 0.1% (=1 g/l)   1.938, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.51
This classifies the protein as unstable.



Aliphatic index: 57.83

Grand average of hydropathicity (GRAVY): -0.896