ProtParam
User-provided sequence:
10 20 30 40 50 60
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV
70 80 90 100 110 120
DCFTKYCVTG SADYSIKLWD VSNGQCVATW KSPVPVKRVE FSPCGNYFLA ILDNVMKNPG
130 140 150 160 170 180
SINIYEIERD SATHELTKVS EEPIHKIITH EGLDAATVAG WSTKGKYIIA GHKDGKISKY
190 200 210 220 230 240
DVSNNYEYVD SIDLHEKSIS DMQFSPDLTY FITSSRDTNS FLVDVSTLQV LKKYETDCPL
250 260 270 280 290 300
NTAVITPLKE FIILGGGQEA KDVTTTSANE GKFEARFYHK IFEEEIGRVQ GHFGPLNTVA
310 320 330 340
ISPQGTSYAS GGEDGFIRLH HFEKSYFDFK YDVEKAAEAK EHMQEAN
References and
documentation are available.
Number of amino acids: 347
Molecular weight: 38755.44
Theoretical pI: 5.41
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1733
Hydrogen H 2656
Nitrogen N 450
Oxygen O 540
Sulfur S 10
Formula: C
1733H
2656N
450O
540S
10
Total number of atoms: 5389
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51715
Abs 0.1% (=1 g/l) 1.334, assuming all pairs of Cys residues form cystines
Ext. coefficient 51340
Abs 0.1% (=1 g/l) 1.325, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.51
This classifies the protein as stable.
Aliphatic index: 75.85
Grand average of hydropathicity (GRAVY): -0.404