ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG HTGTIWSIDV 

        70         80         90        100        110        120 
DCFTKYCVTG SADYSIKLWD VSNGQCVATW KSPVPVKRVE FSPCGNYFLA ILDNVMKNPG 

       130        140        150        160        170        180 
SINIYEIERD SATHELTKVS EEPIHKIITH EGLDAATVAG WSTKGKYIIA GHKDGKISKY 

       190        200        210        220        230        240 
DVSNNYEYVD SIDLHEKSIS DMQFSPDLTY FITSSRDTNS FLVDVSTLQV LKKYETDCPL 

       250        260        270        280        290        300 
NTAVITPLKE FIILGGGQEA KDVTTTSANE GKFEARFYHK IFEEEIGRVQ GHFGPLNTVA 

       310        320        330        340 
ISPQGTSYAS GGEDGFIRLH HFEKSYFDFK YDVEKAAEAK EHMQEAN 

References and documentation are available.

Number of amino acids: 347

Molecular weight: 38755.44

Theoretical pI: 5.41

Amino acid composition: 
Ala (A)  20	  5.8%
Arg (R)   8	  2.3%
Asn (N)  14	  4.0%
Asp (D)  23	  6.6%
Cys (C)   6	  1.7%
Gln (Q)   8	  2.3%
Glu (E)  26	  7.5%
Gly (G)  26	  7.5%
His (H)  12	  3.5%
Ile (I)  24	  6.9%
Leu (L)  22	  6.3%
Lys (K)  28	  8.1%
Met (M)   4	  1.2%
Phe (F)  16	  4.6%
Pro (P)  11	  3.2%
Ser (S)  31	  8.9%
Thr (T)  25	  7.2%
Trp (W)   5	  1.4%
Tyr (Y)  16	  4.6%
Val (V)  22	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1733
Hydrogen    H	      2656
Nitrogen    N	       450
Oxygen      O	       540
Sulfur      S	        10

Formula: C1733H2656N450O540S10
Total number of atoms: 5389

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51715
Abs 0.1% (=1 g/l)   1.334, assuming all pairs of Cys residues form cystines


Ext. coefficient    51340
Abs 0.1% (=1 g/l)   1.325, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.51
This classifies the protein as stable.



Aliphatic index: 75.85

Grand average of hydropathicity (GRAVY): -0.404