ProtParam
User-provided sequence:
10 20 30 40 50 60
MKALILVGGF GTRLRPLTLS FPKPLVDFAN KPMILHQIEA LKAVGVDEVV LAINYQPEVM
70 80 90 100 110 120
LNFLKDFETK LEIKITCSQE TEPLGTAGPL ALARDKLLDG SGEPFFVLNS DVISEYPLKE
130 140 150 160 170 180
MLEFHKSHGG EASIMVTKVD EPSKYGVVVM EESTGRVEKF VEKPKLYVGN KINAGIYLLN
190 200 210 220 230 240
PSVLDKIELR PTSIEKETFP KIAAAQGLYA MVLPGFWMDI GQPRDYITGL RLYLDSLRKK
250 260 270 280 290 300
SPAKLTSGPH IVGNVLVDET ATIGEGCLIG PDVAIGPGCI VESGVRLSRC TVMRGVRIKK
310 320 330 340 350 360
HACISSSIIG WHSTVGQWAR IENMTILGED VHVSDEIYSN GGVVLPHKEI KSNILKPEIV
M
References and
documentation are available.
Number of amino acids: 361
Molecular weight: 39577.28
Theoretical pI: 6.28
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1786
Hydrogen H 2881
Nitrogen N 463
Oxygen O 514
Sulfur S 16
Formula: C
1786H
2881N
463O
514S
16
Total number of atoms: 5660
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30160
Abs 0.1% (=1 g/l) 0.762, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.756, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.18
This classifies the protein as stable.
Aliphatic index: 105.24
Grand average of hydropathicity (GRAVY): 0.067