ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKALWALLLV PLLTGCLAEG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV QTLPDQVQEE 

        70         80         90        100        110        120 
LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR ARLAKEVQAA QARLGADMED 

       130        140        150        160        170        180 
LRNRLGQYRN EVNTMLGQST EELRSRLSTH LRKMRKRLMR DADDLQKRLA VYKAGAQEGA 

       190        200        210        220        230        240 
ERGVSAIRER LGPLVEQGRQ RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE 

       250        260        270        280        290        300 
EVREQMEEVR SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN 

       310 
SIASTTVPLE NQ 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 35763.49

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  31	  9.9%
Arg (R)  32	 10.3%
Asn (N)   9	  2.9%
Asp (D)  14	  4.5%
Cys (C)   1	  0.3%
Gln (Q)  32	 10.3%
Glu (E)  37	 11.9%
Gly (G)  17	  5.4%
His (H)   1	  0.3%
Ile (I)   7	  2.2%
Leu (L)  39	 12.5%
Lys (K)  12	  3.8%
Met (M)  11	  3.5%
Phe (F)   3	  1.0%
Pro (P)   9	  2.9%
Ser (S)  12	  3.8%
Thr (T)  16	  5.1%
Trp (W)   6	  1.9%
Tyr (Y)   4	  1.3%
Val (V)  19	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1537
Hydrogen    H	      2521
Nitrogen    N	       469
Oxygen      O	       488
Sulfur      S	        12

Formula: C1537H2521N469O488S12
Total number of atoms: 5027

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38960
Abs 0.1% (=1 g/l)   1.089, assuming all pairs of Cys residues form cystines


Ext. coefficient    38960
Abs 0.1% (=1 g/l)   1.089, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.67
This classifies the protein as unstable.



Aliphatic index: 85.10

Grand average of hydropathicity (GRAVY): -0.710