ProtParam
User-provided sequence:
10 20 30 40 50 60
MKARKSQRKA GSKPNLIQSK LQVNNGSKSN KIVKCDKCEM SYSSTSIEDR AIHEKYHTLQ
70 80 90 100 110 120
LHGRKWSPNW GSIVYTERNH SRTVHLSRST GTITPLNSSP LKKSSPSITH QEEKIVYVRP
130 140 150 160 170 180
DKSNGEVRAM TEIMTLVNNE LNAPHDENVI WNSTTEEKGK AFVYIRNDRA VGIIIIENLY
190 200 210 220 230 240
GGNGKTSSRG RWMVYDSRRL VQNVYPDFKI GISRIWVCRT ARKLGIATKL IDVARENIVY
250 260 270 280
GEVIPRYQVA WSQPTDSGGK LASKYNGIMH KSGKLLLPVY I
References and
documentation are available.
Number of amino acids: 281
Molecular weight: 31845.46
Theoretical pI: 9.94
Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1402
Hydrogen H 2263
Nitrogen N 415
Oxygen O 414
Sulfur S 9
Formula: C
1402H
2263N
415O
414S
9
Total number of atoms: 4503
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51005
Abs 0.1% (=1 g/l) 1.602, assuming all pairs of Cys residues form cystines
Ext. coefficient 50880
Abs 0.1% (=1 g/l) 1.598, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.97
This classifies the protein as stable.
Aliphatic index: 81.81
Grand average of hydropathicity (GRAVY): -0.603