ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKAVVIEDGK AVVKEGVPIP ELEEGFVLIK TLAVAGNPTD WAHIDYKVGP QGSILGCDAA 

        70         80         90        100        110        120 
GQIVKLGPAV DPKDFSIGDY IYGFIHGSSV RFPSNGAFAE YSAISTVVAY KSPNELKFLG 

       130        140        150        160        170        180 
EDVLPAGPVR SLEGAATIPV SLTTAGLVLT YNLGLNLKWE PSTPQRNGPI LLWGGATAVG 

       190        200        210        220        230        240 
QSLIQLANKL NGFTKIIVVA SRKHEKLLKE YGADQLFDYH DIDVVEQIKH KYNNISYLVD 

       250        260        270        280        290        300 
CVANQNTLQQ VYKCAADKQD ATVVELTNLT EENVKKENRR QNVTIDRTRL YSIGGHEVPF 

       310        320        330        340        350        360 
GGITFPADPE ARRAATEFVK FINPKISDGQ IHHIPARVYK NGLYDVPRIL EDIKIGKNSG 


EKLVAVLN 

References and documentation are available.

Number of amino acids: 368

Molecular weight: 40120.88

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  31	  8.4%
Arg (R)  12	  3.3%
Asn (N)  20	  5.4%
Asp (D)  20	  5.4%
Cys (C)   3	  0.8%
Gln (Q)  13	  3.5%
Glu (E)  22	  6.0%
Gly (G)  32	  8.7%
His (H)   8	  2.2%
Ile (I)  28	  7.6%
Leu (L)  32	  8.7%
Lys (K)  27	  7.3%
Met (M)   1	  0.3%
Phe (F)  12	  3.3%
Pro (P)  20	  5.4%
Ser (S)  17	  4.6%
Thr (T)  18	  4.9%
Trp (W)   3	  0.8%
Tyr (Y)  14	  3.8%
Val (V)  35	  9.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1813
Hydrogen    H	      2869
Nitrogen    N	       483
Oxygen      O	       535
Sulfur      S	         4

Formula: C1813H2869N483O535S4
Total number of atoms: 5704

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37485
Abs 0.1% (=1 g/l)   0.934, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   0.931, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.89
This classifies the protein as stable.



Aliphatic index: 99.59

Grand average of hydropathicity (GRAVY): -0.125