ProtParam
User-provided sequence:
10 20 30 40 50 60
MKCRVWSEAR VYTNINKQRT EEYWDYENTV IDWSTNTKDY EIENKVGRGK YSEVFQGVKL
70 80 90 100 110 120
DSKVKIVIKM LKPVKKKKIK REIKILTDLS NEKVPPTTLP FQKDQYYTNQ KEDVLKFIRP
130 140 150 160 170 180
YIFDQPHNGH ANIIHLFDII KDPISKTPAL VFEYVDNVDF RILYPKLTDL EIRFYMFELL
190 200 210 220 230 240
KALDYCHSMG IMHRDVKPHN VMIDHKNKKL RLIDWGLAEF YHVNMEYNVR VASRFFKGPE
250 260 270 280 290 300
LLVDYRMYDY SLDLWSFGTM LASMIFKREP FFHGTSNTDQ LVKIVKVLGT SDFEKYLLKY
310 320 330 340 350 360
EITLPREFYD MDQYIRKPWH RFINDGNKHL SGNDEIIDLI DNLLRYDHQE RLTAKEAMGH
370
PWFAPIREQI EK
References and
documentation are available.
Number of amino acids: 372
Molecular weight: 44667.58
Theoretical pI: 8.63
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2047
Hydrogen H 3148
Nitrogen N 532
Oxygen O 563
Sulfur S 14
Formula: C
2047H
3148N
532O
563S
14
Total number of atoms: 6304
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 71405
Abs 0.1% (=1 g/l) 1.599, assuming all pairs of Cys residues form cystines
Ext. coefficient 71280
Abs 0.1% (=1 g/l) 1.596, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.14
This classifies the protein as stable.
Aliphatic index: 85.62
Grand average of hydropathicity (GRAVY): -0.549