ProtParam
User-provided sequence:
10 20 30 40 50 60
MKDDGAPNVA KTTDPPPPKR FPSGRAHSPH GDGEAGKLLH GSVFSKRFFG SFQWEEFACG
70 80 90 100 110 120
CGAAFVNIAV TYPIYKMIFR QMLHGVPITS AFAQLRHEGL GFLYRGMLPP LAQKTISLSI
130 140 150 160 170 180
MFGVFDGTRR YLVEDYRLND YGAKVLAAVV AGSAESILLP FERVQTLLAD SKFHQHFSNT
190 200 210 220 230 240
QNAFRYVVSH HGYRELYRGL EPVFWRNGLS NALFFVLREE ASVRLPKRKS VSTRTVQEFI
250 260 270 280 290 300
AGAVIGASIS TIFYPLNVIK VSLQSEMGQR SEGSWQACKR IYVERDRRIG NFYRGCPFNT
310 320 330
GRSFISWGIM NTAYENLKKL MQQQPPLPLA SE
References and
documentation are available.
Number of amino acids: 332
Molecular weight: 37362.95
Theoretical pI: 9.71
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1695
Hydrogen H 2608
Nitrogen N 470
Oxygen O 463
Sulfur S 12
Formula: C
1695H
2608N
470O
463S
12
Total number of atoms: 5248
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41620
Abs 0.1% (=1 g/l) 1.114, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 1.107, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.31
This classifies the protein as stable.
Aliphatic index: 78.73
Grand average of hydropathicity (GRAVY): -0.192