ProtParam
User-provided sequence:
10 20 30 40 50 60
MKDNLERAKL MVSSTVFSWL LLCLFAVTTS VSVQPTCTFP AIYNFGDSNS DTGGISAAFE
70 80 90 100 110 120
PIRDPYGQGF FHRPTGRDSD GRLTIDFIAE RLGLPYLSAY LNSLGSNFRH GANFATGGST
130 140 150 160 170 180
IRRQNETIFQ YGISPFSLDM QIAQFDQFKA RSALLFTQIK SRYDREKLPR QEEFAKALYT
190 200 210 220 230 240
FDIGQNDLSV GFRTMSVDQL KATIPDIVNH LASAVRNIYQ QGGRTFWVHN TGPFGCLPVN
250 260 270 280 290 300
MFYMGTPAPG YLDKSGCVKA QNEMAMEFNR KLKETVINLR KELTQAAITY VDVYTAKYEM
310 320 330 340 350 360
MSNPKKLGFA NPLKVCCGYH EKYDHIWCGN KGKVNNTEIY GGSCPNPVMA VSWDGVHYTE
370 380
AANKHVADRT LNGLLTDPPV PITRACYRQ
References and
documentation are available.
Number of amino acids: 389
Molecular weight: 43570.62
Theoretical pI: 8.72
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1947
Hydrogen H 2995
Nitrogen N 531
Oxygen O 568
Sulfur S 20
Formula: C
1947H
2995N
531O
568S
20
Total number of atoms: 6061
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49320
Abs 0.1% (=1 g/l) 1.132, assuming all pairs of Cys residues form cystines
Ext. coefficient 48820
Abs 0.1% (=1 g/l) 1.120, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.06
This classifies the protein as stable.
Aliphatic index: 72.98
Grand average of hydropathicity (GRAVY): -0.294