ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKDQDLELEK VRLISLATKL GFDEDSAKKC LDRFVDLYGD DGRDFITVEL CGDDFLAALA 

        70         80         90        100        110        120 
DFEEGTEEWD DIQAIESEAQ GNLAEMFDKS TNPSDNGFDT DDDDDDSRVE VHVIEDSPEP 

       130        140        150        160        170        180 
KKKPEIVELD SSSDLEDVET RFKVPRRSQT CSRSMDYSME DSVSTISGRK PSVQISNKDH 

       190        200        210        220        230        240 
ETPSYEELQA LDDLEFANLV IFGNKVFRPL QHQACRASME RKDCFVLMPT GGGKSLCYQL 

       250        260        270        280        290        300 
PATLKAGVTI VISPLLSLIQ DQIVALNLKF GIPATFLNSQ QTSSQAAAVL QELRRDNPSC 

       310        320        330        340        350        360 
KLLYVTPEKI AGSSSFLETL RCLDRKGLLA GFVVDEAHCV SQWGHDFRPD YRELGCLKQN 

       370        380        390        400        410        420 
FPRVPVMALT ATATESVCQD VLKSLRIPRA PVLKMSFDRI NLKYEVIVKT KEPLKQLQEL 

       430        440        450        460        470        480 
LRDRFKDQSG IVYCLSKSEC VDVAKFLNEK CKVKTVYYHA GVPAKQRVDV QRKWQTGEVR 

       490        500        510        520        530        540 
IVCATIAFGM GIDKADVRFV IHNTLSKAVE SYYQESGRAG RDGLQAQCIC LYQKKDFSRV 

       550        560        570        580        590        600 
VCMLRNGQGR NMDRFKSAMA QAKKMQQYCE LKTECRRQML LEYFGESFDR MICKSSLNPC 


DNCERS 

References and documentation are available.

Number of amino acids: 606

Molecular weight: 68501.06

Theoretical pI: 5.35

Amino acid composition: 
Ala (A)  38	  6.3%
Arg (R)  38	  6.3%
Asn (N)  17	  2.8%
Asp (D)  52	  8.6%
Cys (C)  23	  3.8%
Gln (Q)  33	  5.4%
Glu (E)  43	  7.1%
Gly (G)  30	  5.0%
His (H)   7	  1.2%
Ile (I)  25	  4.1%
Leu (L)  58	  9.6%
Lys (K)  44	  7.3%
Met (M)  15	  2.5%
Phe (F)  27	  4.5%
Pro (P)  22	  3.6%
Ser (S)  47	  7.8%
Thr (T)  25	  4.1%
Trp (W)   3	  0.5%
Tyr (Y)  15	  2.5%
Val (V)  44	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 82

Atomic composition:

Carbon      C	      2988
Hydrogen    H	      4773
Nitrogen    N	       831
Oxygen      O	       934
Sulfur      S	        38

Formula: C2988H4773N831O934S38
Total number of atoms: 9564

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40225
Abs 0.1% (=1 g/l)   0.587, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.567, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.66
This classifies the protein as unstable.



Aliphatic index: 80.74

Grand average of hydropathicity (GRAVY): -0.411