ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA 

        70         80         90        100        110        120 
ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH 

       130        140        150        160        170        180 
STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG 

       190        200        210        220        230        240 
IIMDSSISKQ ALSEIETRHS EIIKLENRLR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA 

       250        260        270        280 
VDYVERAVSD TKKAVKYQSK ARRKKIMIII CCVILGIIIA CTIGGIFG 

References and documentation are available.

Number of amino acids: 288

Molecular weight: 33152.65

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  16	  5.6%
Arg (R)  23	  8.0%
Asn (N)   8	  2.8%
Asp (D)  22	  7.6%
Cys (C)   4	  1.4%
Gln (Q)  11	  3.8%
Glu (E)  35	 12.2%
Gly (G)  11	  3.8%
His (H)   5	  1.7%
Ile (I)  29	 10.1%
Leu (L)  17	  5.9%
Lys (K)  23	  8.0%
Met (M)  12	  4.2%
Phe (F)   8	  2.8%
Pro (P)   3	  1.0%
Ser (S)  24	  8.3%
Thr (T)  16	  5.6%
Trp (W)   0	  0.0%
Tyr (Y)   5	  1.7%
Val (V)  16	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1422
Hydrogen    H	      2341
Nitrogen    N	       409
Oxygen      O	       467
Sulfur      S	        16

Formula: C1422H2341N409O467S16
Total number of atoms: 4655

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7700
Abs 0.1% (=1 g/l)   0.232, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.225, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.11
This classifies the protein as unstable.



Aliphatic index: 83.96

Grand average of hydropathicity (GRAVY): -0.608