ProtParam
User-provided sequence:
10 20 30 40 50 60
MKDSMSILTQ TPSEPNAAHP QLHHHLSTLQ QQHHQHHLHY GLQPPAVAHS IHSTTTMSSG
70 80 90 100 110 120
GSTTTASGIG KPNRSRFMIN DILAGSAAAA FYKQQQHHQQ LHHHNNNNNS GSSGGSSPAH
130 140 150 160 170 180
SNNNNNINGD NCEASNVAGV GVLPSALHHP QPHPPTHPHT HPHALMHPHG KLGHFPPTAG
190 200 210 220 230 240
GNGLNVAQYA AAMQQHYAAA AAAAAARNNA AAAAAAAAAA AAAGVAAPPV DGGVDGGVGL
250 260 270 280 290 300
APPAGGDLDD SSDYHEENED CDSGNMDDHS VCSNGGKDDD GNSVKSGSTS DMSGLSKKQR
310 320 330 340 350 360
KARTAFTDHQ LQTLEKSFER QKYLSVQERQ ELAHKLDLSD CQVKTWYQNR RTKWKRQTAV
370 380 390 400 410 420
GLELLAEAGN FAAFQRLYGG SPYLGAWPYA AAAGAAHGAT PHTNIDIYYR QAAAAAAMQK
430 440 450 460 470 480
PLPYNLYAGV PSVGVGVGVG VGPAPFSHLS ASSSLSSLSS YYQSAAAAAS AANPGGPHPV
490 500 510 520 530 540
APPPSVGGGS PPSGLVKPIP AHSASASPPP RPPSTPSPTL NPGSPPGRSV DSCSQSDDED
QIQV
References and
documentation are available.
Number of amino acids: 544
Molecular weight: 56083.41
Theoretical pI: 6.67
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 2416
Hydrogen H 3714
Nitrogen N 742
Oxygen O 780
Sulfur S 14
Formula: C
2416H
3714N
742O
780S
14
Total number of atoms: 7666
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40590
Abs 0.1% (=1 g/l) 0.724, assuming all pairs of Cys residues form cystines
Ext. coefficient 40340
Abs 0.1% (=1 g/l) 0.719, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.38
This classifies the protein as unstable.
Aliphatic index: 60.13
Grand average of hydropathicity (GRAVY): -0.552