ProtParam
User-provided sequence:
10 20 30 40 50 60
MKEDMEVLSL ASLPVGFRFS PTDEELVRYY LRLKINGHDN DVRVIREIDI CKWEPWDLPD
70 80 90 100 110 120
FSVVKTTDSE WLFFCPLDRK YPSGSRMNRA TVAGYWKATG KDRKIKSGKT KIIGVKRTLV
130 140 150 160 170 180
FYTGRAPKGT RTCWIMHEYR ATEKDLDGTK SGQNPFVVCK LFKKQDIVNG AAEPEESKSC
190 200 210 220 230 240
EVEPAVSSPT VVDEVEMSEV SPVFPKTEET NPCDVAESSL VIPSECRSGY SVPEVTTTGL
250 260 270 280 290 300
DDIDWLSFME FDSPKLFSPL HSQVQSELGS SFNGLQSESS ELFKNHNEDY IQTQYGTNDA
310 320 330 340 350 360
DEYMSKFLDS FLDIPYEPEQ IPYEPQNLSS CNKINDESKR GIKIRARRAQ APGCAEQFVM
370 380 390 400 410 420
QGDASRRLRL QVNLNSHKSE TDSTQLQFIK KEVKDTTTET MTKGCGNFTR SKSRTSFIFK
430 440 450
KIAAMGCSYR GLFRVGVVAV VCVMSVCSLV A
References and
documentation are available.
Number of amino acids: 451
Molecular weight: 50890.67
Theoretical pI: 5.80
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2240
Hydrogen H 3519
Nitrogen N 607
Oxygen O 698
Sulfur S 24
Formula: C
2240H
3519N
607O
698S
24
Total number of atoms: 7088
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53120
Abs 0.1% (=1 g/l) 1.044, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 1.029, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.78
This classifies the protein as unstable.
Aliphatic index: 69.96
Grand average of hydropathicity (GRAVY): -0.480