ProtParam
User-provided sequence:
10 20 30 40 50 60
MKEIAMRNSK RKPEPTPFAG KKLRSTRLRR KRAQISPVLV QSPLWSKQIG VSAASVDSCS
70 80 90 100 110 120
DLLADDNVSC GSSRVEKSSN PKKTLIEEVE VSKPGYNVKE TIGDSKFRRI TRSYSKLHKE
130 140 150 160 170 180
KEGDEIEVSE SSCVDSNSGA GLRRLNVKGN KINDNDEISF SRSDVTFAGH VSNSRSLNFE
190 200 210 220 230 240
SENKESDVVS VISGVEYCSK FGSVTGGADN EEIEISKPSS FVEADSSLGS AKELKPELEI
250 260 270 280 290 300
VGCVSDLACS EKFSEEVSDS LDDESSEQRS EIYSQYSDFD YSDYTPSIFF DSGSEFSEKS
310 320 330 340 350 360
SSDSPISHSR SLYLQFKEQF CRSTIPNDFG SSCEEEIHSE LLRFDDEEVE ESYLRLRERE
370 380 390 400 410 420
RSHAYMRDCA KAYCSRMDNT GLIPRLRSIM VQWIVKQCSD MGLQQETLFL GVGLLDRFLS
430 440 450 460 470 480
KGSFKSERTL ILVGIASLTL ATRIEENQPY NSIRKRNFTI QNLRYSRHEV VAMEWLVQEV
490 500 510 520 530 540
LNFKCFTPTI FNFLWFYLKA ARANPEVERK AKSLAVTSLS DQTQLCFWPS TVAAALVVLA
550 560 570
CIEHNKISAY QRVIKVHVRT TDNELPECVK SLDWLLGQ
References and
documentation are available.
Number of amino acids: 578
Molecular weight: 65201.21
Theoretical pI: 5.54
Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 76
Atomic composition:
Carbon C 2845
Hydrogen H 4512
Nitrogen N 794
Oxygen O 916
Sulfur S 22
Formula: C
2845H
4512N
794O
916S
22
Total number of atoms: 9089
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56225
Abs 0.1% (=1 g/l) 0.862, assuming all pairs of Cys residues form cystines
Ext. coefficient 55350
Abs 0.1% (=1 g/l) 0.849, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.56
This classifies the protein as unstable.
Aliphatic index: 78.04
Grand average of hydropathicity (GRAVY): -0.483